View source for 5ket


==Structure of the aldo-keto reductase from Coptotermes gestroi==
<StructureSection load='5ket' size='340' side='right'caption='[[5ket]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ket]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coptotermes_gestroi Coptotermes gestroi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KET FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ket FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ket OCA], [https://pdbe.org/5ket PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ket RCSB], [https://www.ebi.ac.uk/pdbsum/5ket PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ket ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A140CVV2_9NEOP A0A140CVV2_9NEOP] 
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== Publication Abstract from PubMed ==
BACKGROUND: In nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps. RESULTS: In the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo-keto reductase (CgAKR-1) were comprehensively investigated. CgAKR-1 displayed major structural differences compared with others AKRs, including the differences in the amino acid composition of the substrate-binding site, providing basis for classification as a founding member of a new AKR subfamily (family AKR1 I). Immunolocalization assays with anti-CgAKR-1 antibodies resulted in strong fluorescence in the salivary gland, proventriculus, and foregut. CgAKR-1 supplementation caused a 32% reduction in phenolic aldehydes, such as furfural, which act as fermentation inhibitors of hemicellulosic hydrolysates, and improved ethanol fermentation by the xylose-fermenting yeast Scheffersomyces stipitis by 45%. We observed synergistic enzymatic interactions between CgAKR-1 and commercial cellulosic cocktail for sugarcane bagasse saccharification, with a maximum synergism degree of 2.17 for sugar release. Our data indicated that additive enzymatic activity could be mediated by reactive oxygen species because CgAKR-1 could produce hydrogen peroxide. CONCLUSION: In summary, we identified the founding member of an AKRI subfamily with a potential role in the termite digestome. CgAKR-1 was found to be a multipurpose enzyme with potential biotechnological applications. The present work provided a basis for the development and application of integrative and multipurpose enzymes in the bioethanol production chain.

The Coptotermes gestroi aldo-keto reductase: a multipurpose enzyme for biorefinery applications.,Tramontina R, Franco Cairo JP, Liberato MV, Mandelli F, Sousa A, Santos S, Rabelo SC, Campos B, Ienczak J, Ruller R, Damasio AR, Squina FM Biotechnol Biofuels. 2017 Jan 3;10:4. doi: 10.1186/s13068-016-0688-6. eCollection, 2017. PMID:28053664<ref>PMID:28053664</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ket" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aldo-keto reductase 3D structures|Aldo-keto reductase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Coptotermes gestroi]]
[[Category: Large Structures]]
[[Category: Campos BM]]
[[Category: Liberato ML]]
[[Category: Squina FM]]
[[Category: Tramontina R]]