View source for 5kj8


==Structure of the Ca2+-bound synaptotagmin-1 SNARE complex (long unit cell form) - from synchrotron diffraction==
<StructureSection load='5kj8' size='340' side='right'caption='[[5kj8]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5kj8]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KJ8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kj8 OCA], [https://pdbe.org/5kj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kj8 RCSB], [https://www.ebi.ac.uk/pdbsum/5kj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kj8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VAMP3_RAT VAMP3_RAT] SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
X-ray free electron lasers (XFELs) reduce the effects of radiation damage on macromolecular diffraction data and thereby extend the limiting resolution. Previously, we adapted classical post-refinement techniques to XFEL diffraction data to produce accurate diffraction data sets from a limited number of diffraction images (Uervirojnangkoorn et al., 2015), and went on to use these techniques to obtain a complete data set from crystals of the synaptotagmin-1 / SNARE complex and to determine the structure at 3.5 A resolution (Zhou et al., 2015). Here, we describe new advances in our methods and present a reprocessed XFEL data set of the synaptotagmin-1 / SNARE complex. The reprocessing produced small improvements in electron density maps and the refined atomic model. The maps also contained more information than those of a lower resolution (4.1 A) synchrotron data set. Processing a set of simulated XFEL diffraction images revealed that our methods yield accurate data and atomic models.

Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex.,Lyubimov AY, Uervirojnangkoorn M, Zeldin OB, Zhou Q, Zhao M, Brewster AS, Michels-Clark T, Holton JM, Sauter NK, Weis WI, Brunger AT Elife. 2016 Oct 12;5. pii: e18740. doi: 10.7554/eLife.18740. PMID:27731796<ref>PMID:27731796</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5kj8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ion channels 3D structures|Ion channels 3D structures]]
*[[Synaptosomal-associated protein|Synaptosomal-associated protein]]
*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
*[[Syntaxin 3D structures|Syntaxin 3D structures]]
*[[Vesicle-associated membrane protein|Vesicle-associated membrane protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Brewster AS]]
[[Category: Brunger AT]]
[[Category: Lyubimov AY]]
[[Category: Sauter NK]]
[[Category: Uervirojnangkoorn M]]
[[Category: Weis WI]]
[[Category: Zhao M]]
[[Category: Zhou Q]]