View source for 5klc


==Structure of CBM_E1, a novel carbohydrate-binding module found by sugar cane soil metagenome==
<StructureSection load='5klc' size='340' side='right'caption='[[5klc]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5klc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KLC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.746&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5klc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5klc OCA], [https://pdbe.org/5klc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5klc RCSB], [https://www.ebi.ac.uk/pdbsum/5klc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5klc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0R5P8X1_9BACT A0A0R5P8X1_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carbohydrate-binding modules (CBMs) are appended to glycoside hydrolases and can contribute to the degradation of complex recalcitrant substrates such as the plant cell wall. For application in bioethanol production, novel enzymes with high catalytic activity against recalcitrant lignocellulosic material are being explored and developed. In this work, we report the functional and structural study of CBM_E1, discovered through a metagenomics approach, which is the founding member of a novel CBM family, CBMxx. CBM_E1, which is linked to an endoglucanase, displayed affinity for mixed linked beta-1,4-beta-1,3-glucans, xyloglucan, avicel and cellooligosaccharides. The crystal structure of CBM_E1 in complex with cellopentaose displayed a canonical beta-sandwich fold comprising two beta sheets. The planar ligand binding site, observed in a parallel orientation with the beta strands, is a typical feature of Type A CBMs. On the other hand, affinity for bacterial crystalline cellulose was not detected, a ligand recognized by Type A CBMs, while binding to soluble glucans was enthalpically driven, typical of Type B modules. These unique properties of CBM_E1 are at the interface between Type A and Type B CBMs.

A novel carbohydrate-binding module from sugar cane soil metagenome featuring unique structural and carbohydrate affinity properties.,Campos BM, Liberato MV, Alvarez TM, Zanphorlin LM, Ematsu GC, Barud H, Polikarpov I, Ruller R, Gilbert HJ, Zeri AC, Squina FM J Biol Chem. 2016 Sep 12. pii: jbc.M116.744383. PMID:27621314<ref>PMID:27621314</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5klc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Uncultured bacterium]]
[[Category: Campos BM]]
[[Category: Liberato MV]]
[[Category: Squina FM]]
[[Category: Zeri ACM]]