View source for 5l24


==Structure of CNTnw N149L in the intermediate 2 state==
<StructureSection load='5l24' size='340' side='right'caption='[[5l24]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5l24]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_wadsworthii_9715 Neisseria wadsworthii 9715]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L24 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6ZL:2-{[(4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSYL)OXY]METHYL}-2-OCTYLDECYL+4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>6ZL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l24 OCA], [https://pdbe.org/5l24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l24 RCSB], [https://www.ebi.ac.uk/pdbsum/5l24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l24 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G4CRQ5_9NEIS G4CRQ5_9NEIS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion.

Visualizing multistep elevator-like transitions of a nucleoside transporter.,Hirschi M, Johnson ZL, Lee SY Nature. 2017 Apr 17. doi: 10.1038/nature22057. PMID:28424521<ref>PMID:28424521</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5l24" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria wadsworthii 9715]]
[[Category: Hirschi M]]
[[Category: Johnson ZL]]
[[Category: Lee S-Y]]