View source for 5lc0


==Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor==
<StructureSection load='5lc0' size='340' side='right'caption='[[5lc0]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5lc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LC0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6T8:N-((S)-3-(4-(AMINOMETHYL)PHENYL)-1-(((R)-4-GUANIDINO-1-(5-HYDROXY-1,3,2-DIOXABORINAN-2-YL)BUTYL)AMINO)-1-OXOPROPAN-2-YL)BENZAMIDE'>6T8</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc0 OCA], [https://pdbe.org/5lc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lc0 RCSB], [https://www.ebi.ac.uk/pdbsum/5lc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A140DLX4_ZIKV A0A140DLX4_ZIKV] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ongoing Zika virus (ZIKV) outbreak is linked to severe neurological disorders. ZIKV relies on its NS2B/NS3 protease for polyprotein processing; hence, this enzyme is an attractive drug target. The 2.7 A crystal structure of ZIKV protease in complex with a peptidomimetic boronic-acid inhibitor reveals a cyclic diester between the boronic acid and glycerol. The P2 4-aminomethylphenylalanine moiety of the inhibitor forms a salt-bridge with the non-conserved Asp83 of NS2B, Ion-pairing between Asp83 and the P2 residue of the substrate likely accounts for the enzyme's high catalytic efficiency. The unusual dimer of the ZIKV protease:inhibitor complex seen in the crystal may provide a model for assemblies formed at high local concentrations of protease at the endoplasmatic reticulum membrane, the site of polyprotein processing.

Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor.,Lei J, Hansen G, Nitsche C, Klein CD, Zhang L, Hilgenfeld R Science. 2016 Jul 7. pii: aag2419. PMID:27386922<ref>PMID:27386922</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5lc0" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus protease 3D structures|Virus protease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zika virus]]
[[Category: Hansen G]]
[[Category: Hilgenfeld R]]
[[Category: Lei J]]
[[Category: Zhang LL]]