View source for 5leg


==Structure of the bacterial sex F pilus (pED208)==
<SX load='5leg' size='340' side='right' viewer='molstar' caption='[[5leg]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5leg]] is a 80 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhi Salmonella enterica subsp. enterica serovar Typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LEG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5leg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5leg OCA], [https://pdbe.org/5leg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5leg RCSB], [https://www.ebi.ac.uk/pdbsum/5leg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5leg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PIL1_SALTI PIL1_SALTI] Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (By similarity).
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== Publication Abstract from PubMed ==
Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 A resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.

Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.,Costa TR, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G Cell. 2016 Sep 8;166(6):1436-1444.e10. doi: 10.1016/j.cell.2016.08.025. PMID:27610568<ref>PMID:27610568</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhi]]
[[Category: Costa TRD]]
[[Category: Egelman EH]]
[[Category: Ilangovan I]]
[[Category: Redzej A]]
[[Category: Santini JM]]
[[Category: Smith TK]]
[[Category: Ukleja M]]
[[Category: Waksman G]]