View source for 5ls8


==Light-activated ruthenium complex bound to a DNA quadruplex==
<StructureSection load='5ls8' size='340' side='right'caption='[[5ls8]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ls8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LS8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=QHL:delta-[Ru(tap2-dppz-CN]2+'>QHL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ls8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ls8 OCA], [https://pdbe.org/5ls8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ls8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ls8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ls8 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
By using X-ray crystallography, we show that the complexes Lambda/Delta-[Ru(TAP)(2) (11-CN-dppz)](2+) (TAP=1,4,5,8-tetraazaphenanthrene, dppz=dipyridophenazine) bind DNA G-quadruplex in an enantiospecific manner that parallels the specificity of these complexes with duplex DNA. The Lambda complex crystallises with the normally parallel stranded d(TAGGGTTA) tetraplex to give the first such antiparallel strand assembly in which syn-guanosine is adjacent to the complex at the 5' end of the quadruplex core. SRCD measurements confirm that the same conformational switch occurs in solution. The Delta enantiomer, by contrast, is present in the structure but stacked at the ends of the assembly. In addition, we report the structure of Lambda-[Ru(phen)(2) (11-CN-dppz)](2+) bound to d(TCGGCGCCGA), a duplex-forming sequence, and use both structural models to provide insight into the motif-specific luminescence response of the isostructural phen analogue enantiomers.

Structural Studies Reveal Enantiospecific Recognition of a DNA G-Quadruplex by a Ruthenium Polypyridyl Complex.,McQuaid K, Abell H, Gurung SP, Allan DR, Winter G, Sorensen T, Cardin DJ, Brazier JA, Cardin CJ, Hall JP Angew Chem Int Ed Engl. 2019 Jul 15;58(29):9881-9885. doi: , 10.1002/anie.201814502. Epub 2019 Jun 12. PMID:30958918<ref>PMID:30958918</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ls8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Abell H]]
[[Category: Cardin CJ]]
[[Category: Hall JP]]
[[Category: McQuaid KT]]