View source for 5lsn


==SINEB2 element of the long non-coding RNA activator of translation AS Uchl1==
<StructureSection load='5lsn' size='340' side='right'caption='[[5lsn]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5lsn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LSN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsn OCA], [https://pdbe.org/5lsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lsn RCSB], [https://www.ebi.ac.uk/pdbsum/5lsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsn ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pervasive transcription of mammalian genomes leads to a previously underestimated level of complexity in gene regulatory networks. Recently, we have identified a new functional class of natural and synthetic antisense long non-coding RNAs (lncRNA) that increases translation of partially overlapping sense mRNAs. These molecules were named SINEUPs, as they require an embedded inverted SINE B2 element for their UP-regulation of translation. Mouse AS Uchl1 is the representative member of natural SINEUPs. It was originally discovered for its role in increasing translation of Uchl1 mRNA, a gene associated with neurodegenerative diseases. Here we present the secondary structure of the SINE B2 Transposable Element (TE) embedded in AS Uchl1. We find that specific structural regions, containing a short hairpin, are required for the ability of AS Uchl1 RNA to increase translation of its target mRNA. We also provide a high-resolution structure of the relevant hairpin, based on NMR observables. Our results highlight the importance of structural determinants in embedded TEs for their activity as functional domains in lncRNAs.

Structural determinants of the SINE B2 element embedded in the long non-coding RNA activator of translation AS Uchl1.,Podbevsek P, Fasolo F, Bon C, Cimatti L, Reisser S, Carninci P, Bussi G, Zucchelli S, Plavec J, Gustincich S Sci Rep. 2018 Feb 16;8(1):3189. doi: 10.1038/s41598-017-14908-6. PMID:29453387<ref>PMID:29453387</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5lsn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Plavec J]]
[[Category: Podbevsek P]]