View source for 5luz


==Structure of Human Neurolysin (E475Q) in complex with neurotensin peptide products==
<StructureSection load='5luz' size='340' side='right'caption='[[5luz]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5luz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LUZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5luz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5luz OCA], [https://pdbe.org/5luz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5luz RCSB], [https://www.ebi.ac.uk/pdbsum/5luz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5luz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NEUL_HUMAN NEUL_HUMAN] Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Proteolysis plays an important role in mitochondrial biogenesis, from the processing of newly imported precursor proteins to the degradation of mitochondrial targeting peptides. Disruption of peptide degradation activity in yeast, plant and mammalian mitochondria is known to have deleterious consequences for organism physiology, highlighting the important role of mitochondrial peptidases. In the present work, we show that the human mitochondrial peptidase neurolysin (hNLN) can degrade mitochondrial presequence peptides as well as other fragments up to 19 amino acids long. The crystal structure of hNLN(E475Q) in complex with the products of neurotensin cleavage at 2.7A revealed a closed conformation with an internal cavity that restricts substrate length and highlighted the mechanism of enzyme opening/closing that is necessary for substrate binding and catalytic activity. Analysis of peptide degradation in vitro showed that hNLN cooperates with presequence protease (PreP or PITRM1) in the degradation of long targeting peptides and amyloid-beta peptide, Abeta1-40, associated with Alzheimer disease, particularly cleaving the hydrophobic fragment Abeta35-40. These findings suggest that a network of proteases may be required for complete degradation of peptides localized in mitochondria.

Mechanism of peptide binding and cleavage by the human mitochondrial peptidase neurolysin.,Teixeira PF, Masuyer G, Pinho CM, Branca RMM, Kmiec B, Wallin C, Warmlander SKTS, Berntsson RP, Ankarcrona M, Graslund A, Lehtio J, Stenmark P, Glaser E J Mol Biol. 2017 Nov 25. pii: S0022-2836(17)30561-2. doi:, 10.1016/j.jmb.2017.11.011. PMID:29183787<ref>PMID:29183787</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5luz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Berntsson RP-A]]
[[Category: Glaser E]]
[[Category: Kmiec B]]
[[Category: Masuyer G]]
[[Category: Stenmark P]]
[[Category: Teixeira PF]]