View source for 5m2s


==R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin==
<StructureSection load='5m2s' size='340' side='right'caption='[[5m2s]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5m2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens] and [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens_FD-1 Ruminococcus flavefaciens FD-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M2S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2s OCA], [https://pdbe.org/5m2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m2s RCSB], [https://www.ebi.ac.uk/pdbsum/5m2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0AEF3_RUMFL A0AEF3_RUMFL] 
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== Publication Abstract from PubMed ==
ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.,Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LM, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CM Sci Rep. 2017 Apr 7;7(1):759. doi: 10.1038/s41598-017-00919-w. PMID:28389644<ref>PMID:28389644</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5m2s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ruminococcus flavefaciens]]
[[Category: Ruminococcus flavefaciens FD-1]]
[[Category: Bule P]]
[[Category: Carvalho AL]]
[[Category: Fontes CMGA]]
[[Category: Najmudin S]]