View source for 5m5c


==Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins==
<SX load='5m5c' size='340' side='right' viewer='molstar' caption='[[5m5c]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5m5c]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M5C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m5c OCA], [https://pdbe.org/5m5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m5c RCSB], [https://www.ebi.ac.uk/pdbsum/5m5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m5c ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/CAMP1_HUMAN CAMP1_HUMAN] Probable microtubule-binding protein that plays a role in the regulation of cell morphology and cytoskeletal organization. Through interaction with spectrin may regulate neurite outgrowth.<ref>PMID:19508979</ref> <ref>PMID:21834987</ref> <ref>PMID:24117850</ref> 
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== Publication Abstract from PubMed ==
CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize noncentrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we found that the CAMSAP C-terminal CKK domain is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncovered how mammalian CKK binds between two tubulin dimers at the interprotofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high-resolution fluorescence microscopy and cryo-electron tomography suggested that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the interprotofilament interface at this site serve as the basis for CKK's minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends.

A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.,Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, Akhmanova A Nat Struct Mol Biol. 2017 Oct 9. doi: 10.1038/nsmb.3483. PMID:28991265<ref>PMID:28991265</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5m5c" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</SX>
[[Category: Bos taurus]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Akhmanova A]]
[[Category: Atherton J]]
[[Category: Baldus M]]
[[Category: Grant BJ]]
[[Category: Houben K]]
[[Category: Hua S]]
[[Category: Jiang K]]
[[Category: Joseph A-P]]
[[Category: Luo Y]]
[[Category: Moores CA]]
[[Category: Roberts AJ]]
[[Category: Scarabelli G]]
[[Category: Stangier MM]]
[[Category: Steinmetz MO]]
[[Category: Topf M]]
[[Category: Van Hooff JJE]]