View source for 5mji


==Crystal Structure of RosB with bound intermediate OHC-RP (8-demethyl-8-formylriboflavin-5'-phosphate)==
<StructureSection load='5mji' size='340' side='right' caption='[[5mji]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5mji]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7O6:[(2~{S},3~{R},4~{R})-5-[8-METHANOYL-7-METHYL-2,4-BIS(OXIDANYLIDENE)-1~{H}-BENZO[G]PTERIDIN-10-IUM-10-YL]-2,3,4-TRIS(OXIDANYL)PENTYL]+DIHYDROGEN+PHOSPHATE'>7O6</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mji OCA], [http://pdbe.org/5mji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mji RCSB], [http://www.ebi.ac.uk/pdbsum/5mji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mji ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
8-demethyl-8-aminoriboflavin-5'-phosphate (AFP) synthase (RosB) catalyzes the key reaction of roseoflavin biosynthesis by forming AFP from riboflavin-5'-phosphate (RP) and glutamate via the intermediates 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) and 8-demethyl-8-carboxylriboflavin-5'-phosphate (HO2 C-RP). To understand this reaction in which a methyl substituent of an aromatic ring is replaced by an amine we structurally characterized RosB in complex with OHC-RP (2.0 A) and AFP (1.7 A). RosB is composed of four flavodoxin-like subunits which have been upgraded with specific extensions and a unique C-terminal arm. It appears that RosB has evolved from an electron- or hydride-transferring flavoprotein to a sophisticated multi-step enzyme which uses RP as a substrate (and not as a cofactor). Structure-based active site analysis was complemented by mutational and isotope-based mass-spectrometric data to propose an enzymatic mechanism on an atomic basis.

The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes.,Konjik V, Brunle S, Demmer U, Vanselow A, Sandhoff R, Ermler U, Mack M Angew Chem Int Ed Engl. 2016 Dec 16. doi: 10.1002/anie.201610292. PMID:27981706<ref>PMID:27981706</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5mji" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bruenle, S]]
[[Category: Demmer, U]]
[[Category: Ermler, U]]
[[Category: Konjik, V]]
[[Category: Mack, M]]
[[Category: Sandhoff, R]]
[[Category: Vanselow, A]]
[[Category: Electron transport]]
[[Category: Flavodoxin-like]]
[[Category: Flavoprotein]]
[[Category: Roseoflavin]]