View source for 5mka


==Maltodextrin binding protein MalE1 from L. casei BL23 bound to gamma-cyclodextrin==
<StructureSection load='5mka' size='340' side='right'caption='[[5mka]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5mka]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_casei Lacticaseibacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MKA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MKA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.149&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900042:gamma-cyclodextrin'>PRD_900042</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mka OCA], [https://pdbe.org/5mka PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mka RCSB], [https://www.ebi.ac.uk/pdbsum/5mka PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mka ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B0L7B0_LACCA B0L7B0_LACCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Catabolite repression is a mechanism that enables bacteria to control carbon utilization. As part of this global regulatory network, components of the phosphoenolpyruvate:carbohydrate phosphotransferase system inhibit the uptake of less favorable sugars when a preferred carbon source such as glucose is available. This process is termed inducer exclusion. In bacteria belonging to the phylum Firmicutes, HPr, phosphorylated at serine 46 (P-Ser46-HPr) is the key player but its mode of action is elusive. To address this question at the level of purified protein components, we have chosen a homolog of the Escherichia coli maltose/maltodextrin ATP-binding cassette transporter from Lactobacillus casei (MalE1-MalF1G1K12 ) as a model system. We show that the solute binding protein, MalE1, binds linear and cyclic maltodextrins but not maltose. Crystal structures of MalE1 complexed with these sugars provide a clue why maltose is not a substrate. P-Ser46-HPr inhibited MalE1/maltotetraose-stimulated ATPase activity of the transporter incorporated in proteoliposomes. Furthermore, cross-linking experiments revealed that P-Ser46-HPr contacts the nucleotide-binding subunit, MalK1, in proximity to the Walker A motif. However, P-Ser46-HPr did not block binding of ATP to MalK1. Together, our findings provide first biochemical evidence that P-Ser-HPr arrests the transport cycle by preventing ATP hydrolysis at the MalK1 subunits of the transporter.

Inducer exclusion in Firmicutes: insights into the regulation of a carbohydrate ATP binding cassette transporter from Lactobacillus casei BL23 by the signal transducing protein P-Ser46-HPr.,Homburg C, Bommer M, Wuttge S, Hobe C, Beck S, Dobbek H, Deutscher J, Licht A, Schneider E Mol Microbiol. 2017 Jul;105(1):25-45. doi: 10.1111/mmi.13680. Epub 2017 Apr 18. PMID:28370477<ref>PMID:28370477</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5mka" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lacticaseibacillus casei]]
[[Category: Large Structures]]
[[Category: Beck S]]
[[Category: Bommer M]]
[[Category: Deutscher J]]
[[Category: Dobbek H]]
[[Category: Hobe C]]
[[Category: Homburg C]]
[[Category: Licht A]]
[[Category: Schneider E]]
[[Category: Wuttge S]]