View source for 5mpx


==Crystal structure of Arabidopsis thaliana RNA editing factor MORF1, space group P2(1)==
<StructureSection load='5mpx' size='340' side='right'caption='[[5mpx]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5mpx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MPX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.938&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpx OCA], [https://pdbe.org/5mpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mpx RCSB], [https://www.ebi.ac.uk/pdbsum/5mpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MORF1_ARATH MORF1_ARATH] Involved in organellar RNA editing. Required for the processing of numerous RNA editing sites in mitochondria (PubMed:22411807, PubMed:23818871). Binds to the mitochondrial MEF19 and MEF21 factors, two pentatricopeptide repeat-containing proteins involved in RNA editing (PubMed:22411807).<ref>PMID:22411807</ref> <ref>PMID:23818871</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In flowering plant plastids and mitochondria, multiple organellar RNA editing factor (MORF/RIP) proteins are required at most sites for efficient C to U RNA editing catalyzed by the RNA editosome. MORF proteins harbor a conserved stretch of residues (MORF-box), form homo- and heteromers and interact with selected PPR (pentatricopeptide repeat) proteins, which recognize each editing site. The molecular function of the MORF-box remains elusive since it shares no sequence similarity with known domains. We determined structures of the A. thaliana mitochondrial MORF1 and chloroplast MORF9 MORF-boxes which both adopt a novel globular fold (MORF domain). Our structures state a paradigmatic model for MORF domains and their specific dimerization via a hydrophobic interface. We cross-validate the interface by yeast two-hybrid studies and pulldown assays employing structure-based mutants. We find a structural similarity of the MORF domain to an N-terminal ferredoxin-like domain (NFLD), which confers RNA substrate positioning in bacterial 4-thio-uracil tRNA synthetases, implying direct RNA contacts of MORF proteins during RNA editing. With the MORF1 and MORF9 structures we elucidate a yet unknown fold, corroborate MORF interaction studies, validate the mechanism of MORF multimerization by structure-based mutants and pave the way towards a complete structural characterization of the plant RNA editosome.

Crystal structures of the Arabidopsis thaliana organellar RNA editing factors MORF1 and MORF9.,Haag S, Schindler M, Berndt L, Brennicke A, Takenaka M, Weber G Nucleic Acids Res. 2017 May 5;45(8):4915-4928. doi: 10.1093/nar/gkx099. PMID:28201607<ref>PMID:28201607</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5mpx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Berndt L]]
[[Category: Brennicke A]]
[[Category: Haag S]]
[[Category: Schindler M]]
[[Category: Takenaka M]]
[[Category: Weber G]]