View source for 5muz


==Structure of a C-terminal domain of a reptarenavirus L protein==
<StructureSection load='5muz' size='340' side='right'caption='[[5muz]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5muz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MUZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.776&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5muz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5muz OCA], [https://pdbe.org/5muz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5muz RCSB], [https://www.ebi.ac.uk/pdbsum/5muz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5muz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/J7HBG8_9VIRU J7HBG8_9VIRU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cap-snatching was first discovered in influenza virus. Structures of the involved domains of the influenza virus polymerase, namely the endonuclease in the PA subunit and the cap-binding domain in the PB2 subunit, have been solved. Cap-snatching endonucleases have also been demonstrated at the very N-terminus of the L proteins of mammarena-, orthobunya-, and hantaviruses. However, a cap-binding domain has not been identified in an arena- or bunyavirus L protein so far. We solved the structure of the 326 C-terminal residues of the L protein of California Academy of Sciences virus (CASV), a reptarenavirus, by X-ray crystallography. The individual domains of this 37-kDa fragment (L-Cterm) as well as the domain arrangement are structurally similar to the cap-binding and adjacent domains of influenza virus polymerase PB2 subunit, despite the absence of sequence homology, suggesting a common evolutionary origin. This enabled identification of a region in CASV L-Cterm with similarity to a cap-binding site; however, the typical sandwich of two aromatic residues was missing. Consistent with this, cap-binding to CASV L-Cterm could not be detected biochemically. In addition, we solved the crystal structure of the corresponding endonuclease in the N-terminus of CASV L protein. It shows a typical endonuclease fold with an active site configuration that is essentially identical to that of known mammarenavirus endonuclease structures. In conclusion, we provide evidence for a presumably functional cap-snatching endonuclease in the N-terminus and a degenerate cap-binding domain in the C-terminus of a reptarenavirus L protein. Implications of these findings for the cap-snatching mechanism in arenaviruses are discussed.

Structural insights into reptarenavirus cap-snatching machinery.,Rosenthal M, Gogrefe N, Vogel D, Reguera J, Rauschenberger B, Cusack S, Gunther S, Reindl S PLoS Pathog. 2017 May 15;13(5):e1006400. doi: 10.1371/journal.ppat.1006400. PMID:28505175<ref>PMID:28505175</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5muz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: CAS virus]]
[[Category: Large Structures]]
[[Category: Cusack S]]
[[Category: Gogrefe N]]
[[Category: Gunther S]]
[[Category: Rauschenberger B]]
[[Category: Reguera J]]
[[Category: Reindl S]]
[[Category: Rosenthal M]]
[[Category: Vogel D]]