View source for 5njn


==Roll out the beta-barrel: structure and mechanism of Pac13, a unique nucleoside dehydratase==
<StructureSection load='5njn' size='340' side='right'caption='[[5njn]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5njn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coeruleorubidus Streptomyces coeruleorubidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NJN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5njn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njn OCA], [https://pdbe.org/5njn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5njn RCSB], [https://www.ebi.ac.uk/pdbsum/5njn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5njn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E2EKP5_STRC4 E2EKP5_STRC4] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The uridyl peptide antibiotics (UPAs), of which pacidamycin is a member, have a clinically unexploited mode of action and an unusual assembly. Perhaps the most striking feature of these molecules is the biosynthetically unique 3'-deoxyuridine that they share. This moiety is generated by an unusual, small and monomeric dehydratase, Pac13, which catalyses the dehydration of uridine-5'-aldehyde. Here we report the structural characterisation of Pac13 with a series of ligands, and gain insight into the enzyme's mechanism demonstrating that H42 is critical to the enzyme's activity and that the reaction is likely to proceed via an E1cB mechanism. The resemblance of the 3'-deoxy pacidamycin moiety with the synthetic anti-retrovirals, presents a potential opportunity for the utilisation of Pac13 in the biocatalytic generation of antiviral compounds.

Short and Sweet: Pac13 is a Small, Monomeric Dehydratase that Mediates the Formation of the 3'- Deoxy Nucleoside of Pacidamycins.,Michailidou F, Chung CW, Brown MJB, Bent AF, Naismith JH, Leavens WJ, Lynn SM, Sharma SV, Goss RJM Angew Chem Int Ed Engl. 2017 Aug 8. doi: 10.1002/anie.201705639. PMID:28786545<ref>PMID:28786545</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5njn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces coeruleorubidus]]
[[Category: Bent AF]]
[[Category: Goss RJM]]
[[Category: Michailidou F]]
[[Category: Naismith JH]]