View source for 5nlt


==CvAA9A==
<StructureSection load='5nlt' size='340' side='right'caption='[[5nlt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nlt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Collariella_virescens Collariella virescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NLT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nlt OCA], [https://pdbe.org/5nlt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nlt RCSB], [https://www.ebi.ac.uk/pdbsum/5nlt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nlt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A223GEC9_9PEZI A0A223GEC9_9PEZI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinus similis (LsAA9A) and Collariella virescens (CvAA9A). LsAA9A and CvAA9A cleave a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan. LsAA9A additionally cleaves isolated xylan substrates. The structures of CvAA9A and of LsAA9A bound to cellulosic and non-cellulosic oligosaccharides provide insight into the molecular determinants of their specificity. Spectroscopic measurements reveal differences in copper co-ordination upon the binding of xylan and glucans. LsAA9A activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage. Overall, these data show that AA9 LPMOs can display different apparent substrate specificities dependent upon both productive protein-carbohydrate interactions across a binding surface and also electronic considerations at the copper active site.

Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates.,Simmons TJ, Frandsen KEH, Ciano L, Tryfona T, Lenfant N, Poulsen JC, Wilson LFL, Tandrup T, Tovborg M, Schnorr K, Johansen KS, Henrissat B, Walton PH, Lo Leggio L, Dupree P Nat Commun. 2017 Oct 20;8(1):1064. doi: 10.1038/s41467-017-01247-3. PMID:29057953<ref>PMID:29057953</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5nlt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Collariella virescens]]
[[Category: Large Structures]]
[[Category: Frandsen KEH]]
[[Category: Lo Leggio L]]
[[Category: Poulsen J-CN]]
[[Category: Schnorr K]]
[[Category: Tandrup T]]
[[Category: Tovborg M]]