View source for 5npu


==Inferred ancestral pyruvate decarboxylase==
<StructureSection load='5npu' size='340' side='right'caption='[[5npu]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5npu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NPU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5npu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npu OCA], [https://pdbe.org/5npu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5npu RCSB], [https://www.ebi.ac.uk/pdbsum/5npu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5npu ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a key enzyme in homofermentative metabolism where ethanol is the major product. PDCs are thiamine pyrophosphate- and Mg(2+) ion-dependent enzymes that catalyse the non-oxidative decarboxylation of pyruvate to acetaldehyde and carbon dioxide. As this enzyme class is rare in bacteria, current knowledge of bacterial PDCs is extremely limited. One approach to further the understanding of bacterial PDCs is to exploit the diversity provided by evolution. Ancestral sequence reconstruction (ASR) is a method of computational molecular evolution to infer extinct ancestral protein sequences, which can then be synthesized and experimentally characterized. Through ASR a novel PDC was generated, designated ANC27, that shares only 78% amino-acid sequence identity with its closest extant homologue (Komagataeibacter medellinensis PDC, GenBank accession No. WP_014105323.1), yet is fully functional. Crystals of this PDC diffracted to 3.5 A resolution. The data were merged in space group P3221, with unit-cell parameters a = b = 108.33, c = 322.65 A, and contained two dimers (two tetramer halves) in the asymmetric unit. The structure was solved by molecular replacement using PDB entry 2wvg as a model, and the final R values were Rwork = 0.246 (0.3671 in the highest resolution bin) and Rfree = 0.319 (0.4482 in the highest resolution bin). Comparison with extant bacterial PDCs supports the previously observed correlation between decreased tetramer interface area (and number of interactions) and decreased thermostability.

Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase.,Buddrus L, Andrews ESV, Leak DJ, Danson MJ, Arcus VL, Crennell SJ Acta Crystallogr F Struct Biol Commun. 2018 Mar 1;74(Pt 3):179-186. doi:, 10.1107/S2053230X18002819. Epub 2018 Feb 26. PMID:29497023<ref>PMID:29497023</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5npu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pyruvate decarboxylase|Pyruvate decarboxylase]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Andrews ESV]]
[[Category: Arcus VL]]
[[Category: Buddrus L]]
[[Category: Crennell SJ]]
[[Category: Danson MJ]]
[[Category: Leak DJ]]