View source for 5nqa


==Crystal structure of GalNAc-T4 in complex with the monoglycopeptide 3==
<StructureSection load='5nqa' size='340' side='right'caption='[[5nqa]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nqa]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NQA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nqa OCA], [https://pdbe.org/5nqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nqa RCSB], [https://www.ebi.ac.uk/pdbsum/5nqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nqa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GALT4_HUMAN GALT4_HUMAN] Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The polypeptide GalNAc-transferases (GalNAc-Ts), that initiate mucin-type O-glycosylation, consist of a catalytic and a lectin domain connected by a flexible linker. In addition to recognizing polypeptide sequence, the GalNAc-Ts exhibit unique long-range N- and/or C-terminal prior glycosylation (GalNAc-O-Ser/Thr) preferences modulated by the lectin domain. Here we report studies on GalNAc-T4 that reveal the origins of its unique N-terminal long-range glycopeptide specificity, which is the opposite of GalNAc-T2. The GalNAc-T4 structure bound to a monoglycopeptide shows that the GalNAc-binding site of its lectin domain is rotated relative to the homologous GalNAc-T2 structure, explaining their different long-range preferences. Kinetics and molecular dynamics simulations on several GalNAc-T2 flexible linker constructs show altered remote prior glycosylation preferences, confirming that the flexible linker dictates the rotation of the lectin domain, thus modulating the GalNAc-Ts' long-range preferences. This work for the first time provides the structural basis for the different remote prior glycosylation preferences of the GalNAc-Ts.

The interdomain flexible linker of the polypeptide GalNAc transferases dictates their long-range glycosylation preferences.,Rivas ML, Lira-Navarrete E, Daniel EJP, Companon I, Coelho H, Diniz A, Jimenez-Barbero J, Peregrina JM, Clausen H, Corzana F, Marcelo F, Jimenez-Oses G, Gerken TA, Hurtado-Guerrero R Nat Commun. 2017 Dec 5;8(1):1959. doi: 10.1038/s41467-017-02006-0. PMID:29208955<ref>PMID:29208955</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5nqa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Clausen H]]
[[Category: Coelho H]]
[[Category: Companon I]]
[[Category: Corzana F]]
[[Category: Daniel EJP]]
[[Category: Diniz A]]
[[Category: Gerken TA]]
[[Category: Hurtado-Guerrero R]]
[[Category: Jimenez-Barbero J]]
[[Category: Jimenez-Oses G]]
[[Category: Lira-Navarrete E]]
[[Category: Marcelo F]]
[[Category: Peregrina JM]]
[[Category: De las Rivas M]]