View source for 5nxy


==Crystal structure of OpuAC from B. subtilis in complex with Arsenobetaine==
<StructureSection load='5nxy' size='340' side='right'caption='[[5nxy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nxy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NXY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1Y8:2-(TRIMETHYL-LAMBDA~5~-ARSANYL)ETHANOL'>1Y8</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxy OCA], [https://pdbe.org/5nxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nxy RCSB], [https://www.ebi.ac.uk/pdbsum/5nxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OPUBC_BACSU OPUBC_BACSU] Member of a high affinity multicomponent binding-protein-dependent transport system for choline.<ref>PMID:10216873</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Arsenic, a highly cytotoxic and cancerogenic metalloid, is brought into the biosphere through geochemical sources and anthropogenic activities. A global biogeochemical arsenic biotransformation cycle exists in which inorganic arsenic species are transformed into organoarsenicals, which are subsequently mineralized again into inorganic arsenic compounds. Microorganisms contribute to this biotransformation process greatly and one of the organoarsenicals synthesized and degraded in this cycle is arsenobetaine. Its nitrogen-containing homologue glycine betaine is probably the most frequently used compatible solute on Earth. Arsenobetaine is found in marine and terrestrial habitats and even in deep-sea hydrothermal vent ecosystems. Despite its ubiquitous occurrence, the biological function of arsenobetaine has not been comprehensively addressed. Using Bacillus subtilis as a well-understood platform for the study of microbial osmostress adjustment systems, we ascribe here to arsenobetaine both a protective function against high osmolarity and a cytoprotective role against extremes in low and high growth temperatures. We define a biosynthetic route for arsenobetaine from the precursor arsenocholine that relies on enzymes and genetic regulatory circuits for glycine betaine formation from choline, identify the uptake systems for arsenobetaine and arsenocholine, and describe crystal structures of ligand-binding proteins from the OpuA and OpuB ABC transporters complexed with either arsenobetaine or arsenocholine.

Arsenobetaine: an ecophysiologically important organoarsenical confers cytoprotection against osmotic stress and growth temperature extremes.,Hoffmann T, Warmbold B, Smits SHJ, Tschapek B, Ronzheimer S, Bashir A, Chen C, Rolbetzki A, Pittelkow M, Jebbar M, Seubert A, Schmitt L, Bremer E Environ Microbiol. 2018 Jan;20(1):305-323. doi: 10.1111/1462-2920.13999. Epub, 2017 Dec 7. PMID:29159878<ref>PMID:29159878</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5nxy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Bremer E]]
[[Category: Hofmann T]]
[[Category: Schmitt L]]
[[Category: Smits S]]