View source for 5oap


==Solution NMR structure of DREB2A(255-272) bound to RCD1-RST==
<StructureSection load='5oap' size='340' side='right'caption='[[5oap]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5oap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OAP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oap OCA], [https://pdbe.org/5oap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oap RCSB], [https://www.ebi.ac.uk/pdbsum/5oap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oap ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DRE2A_ARATH DRE2A_ARATH] Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3' (PubMed:11798174). Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription (PubMed:11798174). Promotes the expression of heat stress-inducible genes by contributing to the formation of a heat stress-specific transcriptional complex with NF-Y subunits (e.g. DPB3-1, NF-YA2 and NF-YB3) at the promoter of target genes, thus promoting heat tolerance (PubMed:25490919).<ref>PMID:11798174</ref> <ref>PMID:25490919</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Communication within cells relies on a few protein nodes called hubs, which organize vast interactomes with many partners. Frequently, hub proteins are intrinsically disordered conferring multi-specificity and dynamic communication. Conversely, folded hub proteins may organize networks using disordered partners. In this work, the structure of the RST domain, a unique folded hub, is solved by nuclear magnetic resonance spectroscopy and small-angle X-ray scattering, and its complex with a region of the transcription factor DREB2A is provided through data-driven HADDOCK modeling and mutagenesis analysis. The RST fold is unique, but similar structures are identified in the PAH (paired amphipathic helix), TAFH (TATA-box-associated factor homology), and NCBD (nuclear coactivator binding domain) domains. We designate them as a group the alphaalpha hubs, as they share an alphaalpha-hairpin super-secondary motif, which serves as an organizing platform for malleable helices of varying topology. This allows for partner adaptation, exclusion, and selection. Our findings provide valuable insights into structural features enabling signaling fidelity.

Structure of Radical-Induced Cell Death1 Hub Domain Reveals a Common alphaalpha-Scaffold for Disorder in Transcriptional Networks.,Bugge K, Staby L, Kemplen KR, O'Shea C, Bendsen SK, Jensen MK, Olsen JG, Skriver K, Kragelund BB Structure. 2018 Apr 12. pii: S0969-2126(18)30094-7. doi:, 10.1016/j.str.2018.03.013. PMID:29657132<ref>PMID:29657132</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5oap" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Bugge K]]
[[Category: Kragelund BB]]
[[Category: Skriver K]]
[[Category: Staby L]]