View source for 5odf


==NtMe polyamide in complex with 5'CGATGTACATCG3'- hairpin polyamides studies==
<StructureSection load='5odf' size='340' side='right'caption='[[5odf]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5odf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ODF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CWN:NtMe+polyamide'>CWN</scene>, <scene name='pdbligand=DCZ:2-DEOXYCYTIDINE'>DCZ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5odf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odf OCA], [https://pdbe.org/5odf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5odf RCSB], [https://www.ebi.ac.uk/pdbsum/5odf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5odf ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
This manuscript reports the molecular basis for double-stranded DNA (dsDNA) binding of hairpin polyamides incorporating a 5-alkyl thiazole (Nt) unit. Hairpin polyamides containing an N-terminal Nt unit induce higher melting stabilisation of target dsDNA sequences relative to an archetypical hairpin polyamide incorporating an N-terminal imidazole (Im) unit. However, modification of the N-terminus from Im to Nt-building blocks results in an increase in dsDNA binding affinity but lower G-selectivity. A general G-selectivity trend is observed for Nt-containing polyamide analogues. G-selectivity increases as the steric bulk in the Nt 5-position increases. Solution-based NMR structural studies reveal differences in the modulation of the target DNA duplex of Nt-containing hairpin polyamides relative to the Im-containing archetype. A structural hallmark of an Nt polyamide*dsDNA complex is a more significant degree of major groove compression of the target dsDNA sequence relative to the Im-containing hairpin polyamide.

Structural basis of DNA duplex distortion induced by thiazole-containing hairpin polyamides.,Padroni G, Parkinson JA, Fox KR, Burley GA Nucleic Acids Res. 2017 Nov 29. pii: 4670956. doi: 10.1093/nar/gkx1211. PMID:29194552<ref>PMID:29194552</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Burley GA]]
[[Category: Padroni G]]
[[Category: Parkinson J]]