View source for 5ofj


==Crystal structure of N-terminal domain of bifunctional CbXyn10C==
<StructureSection load='5ofj' size='340' side='right'caption='[[5ofj]], [[Resolution|resolution]] 1.34&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ofj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldicellulosiruptor_bescii_DSM_6725 Caldicellulosiruptor bescii DSM 6725]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OFJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.34&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ofj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ofj OCA], [https://pdbe.org/5ofj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ofj RCSB], [https://www.ebi.ac.uk/pdbsum/5ofj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ofj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B9MKT7_CALBD B9MKT7_CALBD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bifunctional glycoside hydrolases have potential for cost saving in enzymatic decomposition of plant cell wall polysaccharides for biofuels and bio-based chemicals. The N-terminal GH10 domain of a bifunctional multimodular enzyme CbXyn10C/Cel48B from Caldicellulosiruptor bescii, is an enzyme able to degrade xylan and cellulose simultaneously. However, the molecular mechanism underlying its substrate promiscuity has not been elucidated. Herein, we discovered that the binding cleft of CbXyn10C would have at least six sugar binding subsites by using isothermal titration calorimetry analysis of the inactive E140Q/E248Q mutant with xylo- and cellooligosaccharides. This was confirmed by determining the catalytic efficiency of the wild-type enzyme on these oligosaccharides. The free form and complex structures of CbXyn10C with xylose- or glucose-configured oligosaccharide ligands were further obtained by crystallographic analysis and molecular modeling and docking. CbXyn10C was found to have a typical (beta/alpha)8-TIM barrel fold and "salad-bowl" shape of GH10 enzymes. In complex structure with xylo-oligosaccharides, seven sugar-binding subsites were found and many residues responsible for substrate interactions were identified. Site-directed mutagenesis indicated that six and ten amino acid residues were key residues for xylan and cellulose hydrolysis, respectively. The most important residues are centered on the subsites -2 and -1 near the cleavage site, while residues playing moderate roles could be located at more distal regions of the binding cleft. Manipulating the residues directly or indirectly interacting with substrates in the distal regions improved the activity of CbXyn10C on xylan and cellulose. Most of the key residues for cellulase activity are conserved across GH10 xylanases. Revisiting randomly selected GH10 enzymes revealed unreported cellulase activity, indicating that the dual function may be a more common phenomenon than has been expected.

Insights into the roles of non-catalytic residues in the active site of a GH10 xylanase with activity on cellulose.,Chu Y, Tu T, Penttinen L, Xue X, Wang X, Yi Z, Gong L, Rouvinen J, Luo H, Hakulinen N, Yao B, Su X J Biol Chem. 2017 Oct 3. pii: jbc.M117.807768. doi: 10.1074/jbc.M117.807768. PMID:28974575<ref>PMID:28974575</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ofj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Caldicellulosiruptor bescii DSM 6725]]
[[Category: Large Structures]]
[[Category: Hakulinen N]]
[[Category: Penttinen L]]
[[Category: Rouvinen J]]