View source for 5ofp


==Structure of the antibacterial peptide ABC transporter McjD in an apo inward occluded conformation==
<StructureSection load='5ofp' size='340' side='right'caption='[[5ofp]], [[Resolution|resolution]] 4.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ofp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OFP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.71&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ofp OCA], [https://pdbe.org/5ofp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ofp RCSB], [https://www.ebi.ac.uk/pdbsum/5ofp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ofp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MCJD_ECOLX MCJD_ECOLX] Is able to protect a cell, which harbors the plasmid pTUC100 encoding microcin J25, against microcin J25. Is required for microcin J25 export out of the producing cells.<ref>PMID:10198038</ref> <ref>PMID:8655570</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Certain pathogenic bacteria produce and release toxic peptides to ensure either nutrient availability or evasion from the immune system. These peptides are also toxic to the producing bacteria that utilize dedicated ABC transporters to provide self-immunity. The ABC transporter McjD exports the antibacterial peptide MccJ25 in Escherichia coli Our previously determined McjD structure provided some mechanistic insights into antibacterial peptide efflux. In this study, we have determined its structure in a novel conformation, apo inward-occluded and a new nucleotide-bound state, high-energy outward-occluded intermediate state, with a defined ligand binding cavity. Predictive cysteine cross-linking in E. coli membranes and PELDOR measurements along the transport cycle indicate that McjD does not undergo major conformational changes as previously proposed for multi-drug ABC exporters. Combined with transport assays and molecular dynamics simulations, we propose a novel mechanism for toxic peptide ABC exporters that only requires the transient opening of the cavity for release of the peptide. We propose that shielding of the cavity ensures that the transporter is available to export the newly synthesized peptides, preventing toxic-level build-up.

Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD.,Bountra K, Hagelueken G, Choudhury HG, Corradi V, El Omari K, Wagner A, Mathavan I, Zirah S, Yuan Wahlgren W, Tieleman DP, Schiemann O, Rebuffat S, Beis K EMBO J. 2017 Oct 16;36(20):3062-3079. doi: 10.15252/embj.201797278. Epub 2017 Sep, 1. PMID:28864543<ref>PMID:28864543</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ofp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Beis K]]
[[Category: Choudhury HG]]