View source for 5opf


==Structure of LPMO10B from from Micromonospora aurantiaca==
<StructureSection load='5opf' size='340' side='right'caption='[[5opf]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5opf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_aurantiaca_ATCC_27029 Micromonospora aurantiaca ATCC 27029]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.081&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opf OCA], [https://pdbe.org/5opf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opf RCSB], [https://www.ebi.ac.uk/pdbsum/5opf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opf ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial lytic polysaccharide monooxygenases (LPMO10s) use redox chemistry to cleave glycosidic bonds in the two foremost recalcitrant polysaccharides found in nature, namely cellulose and chitin. Analysis of correlated mutations revealed that the substrate-binding and copper-containing surface of LPMO10s comprises a network of coevolved residues and interactions, whose roles in LPMO functionality are unclear. Here, we mutated a subset of these correlated residues in a newly characterized C1/C4-oxidizing LPMO10 from Micromonospora aurantiaca (MaLPMO10B) to the corresponding residues in strictly C1-oxidizing LPMO10s. We found that surface properties near the catalytic copper, i.e. side chains likely to be involved in substrate positioning, are major determinants of the C1:C4 ratio. Several MaLPMO10B mutants almost completely lost C4-oxidizing activity while maintaining C1-oxidizing activity. These mutants also lost chitin-oxidizing activity, which is typically observed for C1/C4-oxidizing, but not for C1-oxidizing, cellulose-active LPMO10s. Selective loss in C1-oxidizing activity was not observed. Additional mutational experiments disclosed that neither truncation of MaLPMO10B's family 2 carbohydrate-binding module, nor mutations altering access to the solvent-exposed axial copper coordination site significantly change the C1:C4 ratio. Importantly, several of the mutations that altered interactions with the substrate exhibited reduced stability. This effect could be explained by productive substrate binding that protects LPMOs from oxidative self-inactivation. We discuss these stability issues in view of recent findings on LPMO catalysis, such as the involvement of H2O2 Our results show that residues on the substrate-binding surface of LPMOs have co-evolved to optimize several interconnected properties: substrate binding and specificity, oxidative regioselectivity, catalytic efficiency and stability.

Structural determinants of bacterial lytic polysaccharide monooxygenase functionality.,Forsberg Z, Bissaro B, Gullesen J, Dalhus B, Vaaje-Kolstad G, Eijsink VGH J Biol Chem. 2017 Dec 8. pii: M117.817130. doi: 10.1074/jbc.M117.817130. PMID:29222333<ref>PMID:29222333</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5opf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Micromonospora aurantiaca ATCC 27029]]
[[Category: Bissaro B]]
[[Category: Dalhus B]]
[[Category: Eijsink VGH]]
[[Category: Forsberg Z]]
[[Category: Gullesen J]]
[[Category: Vaaje-Kolstad G]]