View source for 5pwc


==PanDDA analysis group deposition -- Crystal Structure of SP100 in complex with E48115b==
<StructureSection load='5pwc' size='340' side='right'caption='[[5pwc]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5pwc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PWC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.549&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=W77:2,4-DICHLORO-N-(PYRIDIN-3-YL)BENZAMIDE'>W77</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5pwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5pwc OCA], [https://pdbe.org/5pwc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5pwc RCSB], [https://www.ebi.ac.uk/pdbsum/5pwc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5pwc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H0Y4R8_HUMAN H0Y4R8_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In macromolecular crystallography, the rigorous detection of changed states (for example, ligand binding) is difficult unless signal is strong. Ambiguous ('weak' or 'noisy') density is experimentally common, since molecular states are generally only fractionally present in the crystal. Existing methodologies focus on generating maximally accurate maps whereby minor states become discernible; in practice, such map interpretation is disappointingly subjective, time-consuming and methodologically unsound. Here we report the PanDDA method, which automatically reveals clear electron density for the changed state-even from inaccurate maps-by subtracting a proportion of the confounding 'ground state'; changed states are objectively identified from statistical analysis of density distributions. The method is completely general, implying new best practice for all changed-state studies, including the routine collection of multiple ground-state crystals. More generally, these results demonstrate: the incompleteness of atomic models; that single data sets contain insufficient information to model them fully; and that accuracy requires further map-deconvolution approaches.

A multi-crystal method for extracting obscured crystallographic states from conventionally uninterpretable electron density.,Pearce NM, Krojer T, Bradley AR, Collins P, Nowak RP, Talon R, Marsden BD, Kelm S, Shi J, Deane CM, von Delft F Nat Commun. 2017 Apr 24;8:15123. doi: 10.1038/ncomms15123. PMID:28436492<ref>PMID:28436492</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5pwc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Arrowsmith CH]]
[[Category: Bountra C]]
[[Category: Bradley AR]]
[[Category: Brandao-Neto J]]
[[Category: Brennan PE]]
[[Category: Collins P]]
[[Category: Cox O]]
[[Category: Dias A]]
[[Category: Douangamath A]]
[[Category: Edwards A]]
[[Category: Fairhead M]]
[[Category: Krojer T]]
[[Category: MacLean E]]
[[Category: Ng J]]
[[Category: Pearce NM]]
[[Category: Renjie Z]]
[[Category: Sethi R]]
[[Category: Talon R]]
[[Category: Wright N]]
[[Category: Von Delft F]]