View source for 5syq


==Solution structure of Aquifex aeolicus Aq1974==
<StructureSection load='5syq' size='340' side='right'caption='[[5syq]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5syq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2n0p 2n0p]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SYQ FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5syq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5syq OCA], [https://pdbe.org/5syq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5syq RCSB], [https://www.ebi.ac.uk/pdbsum/5syq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5syq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y1974_AQUAE Y1974_AQUAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We determined the NMR structure of a highly aromatic (13%) protein of unknown function, Aq1974 from Aquifex aeolicus (PDB ID: 5SYQ). The unusual sequence of this protein has a tryptophan content five times the normal (six tryptophan residues of 114 or 5.2% while the average tryptophan content is 1.0%) with the tryptophans occurring in a WXW motif. It has no detectable sequence homology with known protein structures. Although its NMR spectrum suggested that the protein was rich in beta-sheet, upon resonance assignment and solution structure determination, the protein was found to be primarily alpha-helical with a small two-stranded beta-sheet with a novel fold that we have termed an Aromatic Claw. As this fold was previously unknown and the sequence unique, we submitted the sequence to CASP10 as a target for blind structural prediction. At the end of the competition, the sequence was classified a hard template based model; the structural relationship between the template and the experimental structure was small and the predictions all failed to predict the structure. CSRosetta was found to predict the secondary structure and its packing; however, it was found that there was little correlation between CSRosetta score and the RMSD between the CSRosetta structure and the NMR determined one. This work demonstrates that even in relatively small proteins, we do not yet have the capacity to accurately predict the fold for all primary sequences. The experimental discovery of new folds helps guide the improvement of structural prediction methods.

Aromatic claw: A new fold with high aromatic content that evades structural prediction.,Sachleben JR, Adhikari AN, Gawlak G, Hoey RJ, Liu G, Joachimiak A, Montelione GT, Sosnick TR, Koide S Protein Sci. 2016 Oct 17. doi: 10.1002/pro.3069. PMID:27750371<ref>PMID:27750371</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5syq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus VF5]]
[[Category: Large Structures]]
[[Category: Gawlak G]]
[[Category: Hoey RJ]]
[[Category: Joachimiak A]]
[[Category: Koide S]]
[[Category: Liu G]]
[[Category: Montelione GT]]
[[Category: Sachleben JR]]