View source for 5tcs


==Crystal structure of a Dwarf Ndc80 Tetramer==
<StructureSection load='5tcs' size='340' side='right'caption='[[5tcs]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tcs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8313&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcs OCA], [https://pdbe.org/5tcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcs RCSB], [https://www.ebi.ac.uk/pdbsum/5tcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NUF2_YEAST NUF2_YEAST] Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a approximately 600 A long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends. The junction connects two alpha-helical coiled coils through regions of four-chain and three-chain overlap. The complexity of its structure depends on interactions among conserved amino-acid residues, suggesting a binding site for additional cellular factor(s) not yet identified.

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.,Valverde R, Ingram J, Harrison SC Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957<ref>PMID:27851957</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5tcs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Harrison SC]]
[[Category: Valverde R]]