View source for 5tgz


==Crystal Structure of the Human Cannabinoid Receptor CB1==
<StructureSection load='5tgz' size='340' side='right'caption='[[5tgz]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tgz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TGZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZDG:4-[4-[2-(2,4-DICHLOROPHENYL)-4-METHYL-5-(PIPERIDIN-1-YLCARBAMOYL)PYRAZOL-3-YL]PHENYL]BUT-3-YNYL+NITRATE'>ZDG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgz OCA], [https://pdbe.org/5tgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tgz RCSB], [https://www.ebi.ac.uk/pdbsum/5tgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CNR1_HUMAN CNR1_HUMAN] Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.<ref>PMID:15620723</ref> [https://www.uniprot.org/uniprot/FLAV_NITV2 FLAV_NITV2] Low-potential electron donor to a number of redox enzymes.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cannabinoid receptor 1 (CB1) is the principal target of Delta9-tetrahydrocannabinol (THC), a psychoactive chemical from Cannabis sativa with a wide range of therapeutic applications and a long history of recreational use. CB1 is activated by endocannabinoids and is a promising therapeutic target for pain management, inflammation, obesity, and substance abuse disorders. Here, we present the 2.8 A crystal structure of human CB1 in complex with AM6538, a stabilizing antagonist, synthesized and characterized for this structural study. The structure of the CB1-AM6538 complex reveals key features of the receptor and critical interactions for antagonist binding. In combination with functional studies and molecular modeling, the structure provides insight into the binding mode of naturally occurring CB1 ligands, such as THC, and synthetic cannabinoids. This enhances our understanding of the molecular basis for the physiological functions of CB1 and provides new opportunities for the design of next-generation CB1-targeting pharmaceuticals.

Crystal Structure of the Human Cannabinoid Receptor CB1.,Hua T, Vemuri K, Pu M, Qu L, Han GW, Wu Y, Zhao S, Shui W, Li S, Korde A, Laprairie RB, Stahl EL, Ho JH, Zvonok N, Zhou H, Kufareva I, Wu B, Zhao Q, Hanson MA, Bohn LM, Makriyannis A, Stevens RC, Liu ZJ Cell. 2016 Oct 20;167(3):750-762.e14. doi: 10.1016/j.cell.2016.10.004. PMID:27768894<ref>PMID:27768894</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5tgz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Desulfovibrio vulgaris str. Hildenborough]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Bohn LM]]
[[Category: Han GW]]
[[Category: Hanson MA]]
[[Category: Ho JH]]
[[Category: Hua T]]
[[Category: Korde A]]
[[Category: Kufareva I]]
[[Category: Laprairie RB]]
[[Category: Li S]]
[[Category: Liu ZJ]]
[[Category: Makriyannis A]]
[[Category: Pu M]]
[[Category: Qu L]]
[[Category: Shui W]]
[[Category: Stahl EL]]
[[Category: Stevens RC]]
[[Category: Vemuri K]]
[[Category: Wu B]]
[[Category: Wu Y]]
[[Category: Zhao Q]]
[[Category: Zhao S]]
[[Category: Zhou H]]
[[Category: Zvonok N]]