View source for 5tis


==Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex==
<StructureSection load='5tis' size='340' side='right'caption='[[5tis]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tis]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TIS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2500038&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=PHO:PHEOPHYTIN+A'>PHO</scene>, <scene name='pdbligand=PL9:2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE'>PL9</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tis OCA], [https://pdbe.org/5tis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tis RCSB], [https://www.ebi.ac.uk/pdbsum/5tis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tis ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSBA1_THEVB PSBA1_THEVB] Photosystem II (PSII) is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.[HAMAP-Rule:MF_01379]<ref>PMID:20558739</ref> <ref>PMID:21367867</ref> <ref>PMID:25006873</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Light-induced oxidation of water by photosystem II (PS II) in plants, algae and cyanobacteria has generated most of the dioxygen in the atmosphere. PS II, a membrane-bound multi-subunit pigment protein complex, couples the one-electron photochemistry at the reaction centre with the four-electron redox chemistry of water oxidation at the Mn4CaO5 cluster in the oxygen-evolving complex (OEC). Under illumination, the OEC cycles through five intermediate S-states (S0 to S4), in which S1 is the dark-stable state and S3 is the last semi-stable state before O-O bond formation and O2 evolution. A detailed understanding of the O-O bond formation mechanism remains a challenge, and will require elucidation of both the structures of the OEC in the different S-states and the binding of the two substrate waters to the catalytic site. Here we report the use of femtosecond pulses from an X-ray free electron laser (XFEL) to obtain damage-free, room temperature structures of dark-adapted (S1), two-flash illuminated (2F; S3-enriched), and ammonia-bound two-flash illuminated (2F-NH3; S3-enriched) PS II. Although the recent 1.95 A resolution structure of PS II at cryogenic temperature using an XFEL provided a damage-free view of the S1 state, measurements at room temperature are required to study the structural landscape of proteins under functional conditions, and also for in situ advancement of the S-states. To investigate the water-binding site(s), ammonia, a water analogue, has been used as a marker, as it binds to the Mn4CaO5 cluster in the S2 and S3 states. Since the ammonia-bound OEC is active, the ammonia-binding Mn site is not a substrate water site. This approach, together with a comparison of the native dark and 2F states, is used to discriminate between proposed O-O bond formation mechanisms.

Structure of photosystem II and substrate binding at room temperature.,Young ID, Ibrahim M, Chatterjee R, Gul S, Fuller FD, Koroidov S, Brewster AS, Tran R, Alonso-Mori R, Kroll T, Michels-Clark T, Laksmono H, Sierra RG, Stan CA, Hussein R, Zhang M, Douthit L, Kubin M, de Lichtenberg C, Vo Pham L, Nilsson H, Cheah MH, Shevela D, Saracini C, Bean MA, Seuffert I, Sokaras D, Weng TC, Pastor E, Weninger C, Fransson T, Lassalle L, Brauer P, Aller P, Docker PT, Andi B, Orville AM, Glownia JM, Nelson S, Sikorski M, Zhu D, Hunter MS, Lane TJ, Aquila A, Koglin JE, Robinson J, Liang M, Boutet S, Lyubimov AY, Uervirojnangkoorn M, Moriarty NW, Liebschner D, Afonine PV, Waterman DG, Evans G, Wernet P, Dobbek H, Weis WI, Brunger AT, Zwart PH, Adams PD, Zouni A, Messinger J, Bergmann U, Sauter NK, Kern J, Yachandra VK, Yano J Nature. 2016 Nov 21. doi: 10.1038/nature20161. PMID:27871088<ref>PMID:27871088</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5tis" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
*[[Photosystem II 3D structures|Photosystem II 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Adams PD]]
[[Category: Afonine PV]]
[[Category: Aller P]]
[[Category: Alonso-Mori R]]
[[Category: Andi B]]
[[Category: Aquila A]]
[[Category: Bean MA]]
[[Category: Bergmann U]]
[[Category: Boutet S]]
[[Category: Braeuer P]]
[[Category: Brewster AS]]
[[Category: Brunger AT]]
[[Category: Chatterjee R]]
[[Category: Cheah MH]]
[[Category: Dobbek H]]
[[Category: Docker PT]]
[[Category: Douthit L]]
[[Category: Evans G]]
[[Category: Fransson T]]
[[Category: Fuller F]]
[[Category: Glownia JM]]
[[Category: Gul S]]
[[Category: Hunter MS]]
[[Category: Hussein R]]
[[Category: Ibrahim M]]
[[Category: Kern J]]
[[Category: Koglin JE]]
[[Category: Koroidov S]]
[[Category: Kroll T]]
[[Category: Kubin M]]
[[Category: Laksmono H]]
[[Category: Lassalle L]]
[[Category: Liang M]]
[[Category: Liebschner D]]
[[Category: Lyubimov AY]]
[[Category: Messinger J]]
[[Category: Michels-Clark T]]
[[Category: Moriarty NW]]
[[Category: Nelson S]]
[[Category: Nilsson H]]
[[Category: Orville AM]]
[[Category: Pastor E]]
[[Category: Pham LV]]
[[Category: Robinson J]]
[[Category: Saracini C]]
[[Category: Sauter NK]]
[[Category: Seuffert I]]
[[Category: Shevela D]]
[[Category: Sierra RG]]
[[Category: Sikorski M]]
[[Category: Sokaras D]]
[[Category: Stan CA]]
[[Category: Tran R]]
[[Category: Uervirojnangkoorn M]]
[[Category: Watermann DG]]
[[Category: Weis WI]]
[[Category: Weng T-C]]
[[Category: Weninger C]]
[[Category: Wernet P]]
[[Category: Yachandra VK]]
[[Category: Yano J]]
[[Category: Young ID]]
[[Category: Zhang M]]
[[Category: Zhu D]]
[[Category: Zouni A]]
[[Category: Zwart PH]]
[[Category: De Lichtenberg C]]