View source for 5tpy


==Crystal structure of an exonuclease resistant RNA from Zika virus==
<StructureSection load='5tpy' size='340' side='right'caption='[[5tpy]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TPY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.805&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tpy OCA], [https://pdbe.org/5tpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tpy RCSB], [https://www.ebi.ac.uk/pdbsum/5tpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tpy ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.

Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease.,Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS Science. 2016 Dec 2;354(6316):1148-1152. Epub 2016 Nov 10. PMID:27934765<ref>PMID:27934765</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5tpy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Art:Zika virus|Art:Zika virus]]
*[[Zika virus subgenomic flaviviral RNA|Zika virus subgenomic flaviviral RNA]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zika virus]]
[[Category: Akiyama BM]]
[[Category: Beckham JD]]
[[Category: Costantino DA]]
[[Category: Kieft JS]]
[[Category: Laurence HM]]
[[Category: Massey AR]]
[[Category: Nix JC]]
[[Category: Shi P-Y]]
[[Category: Xie X]]
[[Category: Yang Y]]