View source for 5tuf


==Crystal structure of tetracycline destructase Tet(50) in complex with anhydrotetracycline==
<StructureSection load='5tuf' size='340' side='right'caption='[[5tuf]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tuf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TUF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tuf OCA], [https://pdbe.org/5tuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tuf RCSB], [https://www.ebi.ac.uk/pdbsum/5tuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tuf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TET50_UNKP TET50_UNKP] An FAD-requiring monooxygenase active on tetracycline antibiotic and some of its derivatives, which leads to their inactivation (PubMed:26097034). Expression in E.coli confers high resistance to tetracycline and oxytetracycline, does not confer resistance to minocycline or tigecycline. Degrades tetracycline and oxytetracycline; the reaction requires NADPH (PubMed:26097034). Degrades and confers resistance to chlortetracycline (PubMed:28481346).<ref>PMID:26097034</ref> <ref>PMID:28481346</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Uncultured bacterium]]
[[Category: Park J]]
[[Category: Tolia NH]]