View source for 5udo


==Crystal structure of the coiled-coil domain from Listeria Innocua Phage Integrase (Tetragonal Form II)==
<StructureSection load='5udo' size='340' side='right'caption='[[5udo]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5udo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_innocua Listeria innocua]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UDO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.541&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5udo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5udo OCA], [https://pdbe.org/5udo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5udo RCSB], [https://www.ebi.ac.uk/pdbsum/5udo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5udo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q928V6_LISIN Q928V6_LISIN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Serine integrases are bacteriophage enzymes that carry out site-specific integration and excision of their viral genomes. The integration reaction is highly directional; recombination between the phage attachment site attP and the host attachment site attB to form the hybrid sites attL and attR is essentially irreversible. In a recent model, extended coiled-coil (CC) domains in the integrase subunits are proposed to interact in a way that favors the attPxattB reaction but inhibits the attLxattR reaction. Here, we show for the Listeria innocua integrase (LI Int) system that the CC domain promotes self-interaction in isolated Int and when Int is bound to attachment sites. Three independent crystal structures of the CC domain reveal the molecular nature of the CC dimer interface. Alanine substitutions of key residues in the interface support the functional significance of the structural model and indicate that the same interaction is responsible for promoting integration and for inhibiting excision. An updated model of a LI Int*attL complex that incorporates the high resolution CC dimer structure provides insights that help to explain the unusual CC dimer structure and potential sources of stability in Int*attL and Int*attR complexes. Together, the data provide a molecular basis for understanding serine integrase directionality.

Coiled-coil interactions mediate serine integrase directionality.,Gupta K, Sharp R, Yuan JB, Li H, Van Duyne GD Nucleic Acids Res. 2017 Jul 7;45(12):7339-7353. doi: 10.1093/nar/gkx474. PMID:28549184<ref>PMID:28549184</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5udo" style="background-color:#fffaf0;"></div>

==See Also==
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Listeria innocua]]
[[Category: Gupta K]]
[[Category: Sharp R]]
[[Category: Van Duyne GD]]
[[Category: Yuan JB]]