View source for 5unq


==Crystal Structure of Pt0534 Inactivated by 2-Oxo-3-pentynoate==
<StructureSection load='5unq' size='340' side='right'caption='[[5unq]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5unq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pusillimonas_sp._T7-7 Pusillimonas sp. T7-7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UNQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.976&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYJ:1-[(2E)-4-carboxy-4-oxobut-2-en-2-yl]-L-proline'>DYJ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5unq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5unq OCA], [https://pdbe.org/5unq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5unq RCSB], [https://www.ebi.ac.uk/pdbsum/5unq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5unq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F4GMX9_PUSST F4GMX9_PUSST] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The tautomerase superfamily (TSF) consists of more than 11,000 non-redundant sequences present throughout the biosphere. Characterized members have attracted much attention because of the unusual and key catalytic role of an N-terminal proline. These few characterized members catalyze a diverse range of chemical reactions, but the full scale of their chemical capabilities and biological functions remains unknown. To gain new insight into TSF structure-function relationships, we performed a global analysis of similarities across the entire superfamily and computed a sequence-similarity network to guide classification into distinct subgroups. Our results indicated that TSF members are found in all domains of life, with most being present in bacteria. The eukaryotic members of the cis-3- chloroacrylic acid dehalogenase subgroup are limited to fungal species, while the macrophagemigration inhibitory factor subgroup has wide eukaryotic representation (including mammals). Unexpectedly, we found that 346 TSF sequences lack Pro-1, of which 85% are present in the malonate semialdehyde decarboxylase subgroup. The computed network also enabled identification of similarity paths, namely sequences that link functionally diverse subgroups and exhibit transitional structural features that may help explain reaction divergence. A structure-guided comparison of these linker proteins identified conserved transitions between them, and kinetic analysis paralleled these observations. Phylogenetic reconstruction of the linker set was consistent with these findings. Our results also suggest that contemporary TSF members may have evolved from a short 4-oxalocrotonate tautomerase-like ancestor, followed by gene duplication and fusion. Our new linker-guided strategy can be used to enrich discovery of sequence/structure/function transitions in other enzyme superfamilies.

A global view of structure-function relationships in the tautomerase superfamily.,Davidson R, Baas BJ, Akiva E, Holliday GL, Polacco BJ, LeVieux JA, Pullara CR, Zhang YJ, Whitman CP, Babbitt PC J Biol Chem. 2017 Nov 28. pii: M117.815340. doi: 10.1074/jbc.M117.815340. PMID:29184004<ref>PMID:29184004</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5unq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pusillimonas sp. T7-7]]
[[Category: Baas BJ]]
[[Category: LeVieux J]]
[[Category: Whitman CP]]
[[Category: Zhang YJ]]