View source for 5uuy


==Crystal structure of Dioclea lasiocarpa lectin (DLL) complexed with X-MAN==
<StructureSection load='5uuy' size='340' side='right'caption='[[5uuy]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5uuy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioclea Dioclea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UUY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uuy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uuy OCA], [https://pdbe.org/5uuy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uuy RCSB], [https://www.ebi.ac.uk/pdbsum/5uuy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uuy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECA_DIOVI LECA_DIOVI] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.[REFERENCE:1]<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lectins are multidomain proteins that specifically recognize various carbohydrates. The structural characterization of these molecules is crucial in understanding their function and activity in systems and organisms. Most cancer cells exhibit changes in glycosylation patterns, and lectins may be able to recognize these changes. In this work, Dioclea lasiocarpa seed lectin (DLL) was structurally characterized. The lectin presented a high degree of similarity with other lectins isolated from legumes, presenting a jelly roll motif and a metal-binding site stabilizing the carbohydrate-recognition domain. DLL demonstrated differential interactions with carbohydrates, depending on type of glycosidic linkage present in ligands. As observed by the reduction of cell viability in C6 cells, DLL showed strong antiglioma activity by mechanisms involving activation of caspase 3.

Structural analysis of Dioclea lasiocarpa lectin: A C6 cells apoptosis-inducing protein.,Nascimento KS, Santiago MQ, Pinto-Junior VR, Osterne VJS, Martins FWV, Nascimento APM, Wolin IAV, Heinrich IA, Martins MGQ, Silva MTL, Lossio CF, Rocha CRC, Leal RB, Cavada BS Int J Biochem Cell Biol. 2017 Sep 20;92:79-89. doi: 10.1016/j.biocel.2017.09.014. PMID:28939357<ref>PMID:28939357</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5uuy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dioclea]]
[[Category: Large Structures]]
[[Category: Cavada BS]]
[[Category: Fonseca FMP]]
[[Category: Nascimento KS]]
[[Category: Neco AHB]]
[[Category: Osterne VJS]]
[[Category: Pinto-Junior VR]]
[[Category: Rocha CRC]]
[[Category: Santiago MQ]]