View source for 5uz7


==Volta phase plate cryo-electron microscopy structure of a calcitonin receptor-heterotrimeric Gs protein complex==
<SX load='5uz7' size='340' side='right' viewer='molstar' caption='[[5uz7]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5uz7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZ7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz7 OCA], [https://pdbe.org/5uz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uz7 RCSB], [https://www.ebi.ac.uk/pdbsum/5uz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref> 
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== Publication Abstract from PubMed ==
Class B G-protein-coupled receptors are major targets for the treatment of chronic diseases, such as osteoporosis, diabetes and obesity. Here we report the structure of a full-length class B receptor, the calcitonin receptor, in complex with peptide ligand and heterotrimeric Galphasbetagamma protein determined by Volta phase-plate single-particle cryo-electron microscopy. The peptide agonist engages the receptor by binding to an extended hydrophobic pocket facilitated by the large outward movement of the extracellular ends of transmembrane helices 6 and 7. This conformation is accompanied by a 60 degrees kink in helix 6 and a large outward movement of the intracellular end of this helix, opening the bundle to accommodate interactions with the alpha5-helix of Galphas. Also observed is an extended intracellular helix 8 that contributes to both receptor stability and functional G-protein coupling via an interaction with the Gbeta subunit. This structure provides a new framework for understanding G-protein-coupled receptor function.

Phase-plate cryo-EM structure of a class B GPCR-G-protein complex.,Liang YL, Khoshouei M, Radjainia M, Zhang Y, Glukhova A, Tarrasch J, Thal DM, Furness SGB, Christopoulos G, Coudrat T, Danev R, Baumeister W, Miller LJ, Christopoulos A, Kobilka BK, Wootten D, Skiniotis G, Sexton PM Nature. 2017 Apr 24. doi: 10.1038/nature22327. PMID:28437792<ref>PMID:28437792</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5uz7" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transducin 3D structures|Transducin 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Homo sapiens]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Baumeister W]]
[[Category: Christopoulos A]]
[[Category: Christopoulos G]]
[[Category: Coudrat T]]
[[Category: Danev R]]
[[Category: Furness SGB]]
[[Category: Glukhova A]]
[[Category: Khoshouei M]]
[[Category: Kobilka BK]]
[[Category: Liang YL]]
[[Category: Miller LJ]]
[[Category: Radjainia M]]
[[Category: Sexton PM]]
[[Category: Skiniotis G]]
[[Category: Tarrasch J]]
[[Category: Thal DM]]
[[Category: Wootten D]]
[[Category: Zhang Y]]