View source for 5uz9


==Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex==
<SX load='5uz9' size='340' side='right' viewer='molstar' caption='[[5uz9]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5uz9]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14], [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD30 Pseudomonas phage JBD30] and [https://en.wikipedia.org/wiki/Pseudomonas_virus_D3112 Pseudomonas virus D3112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZ9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz9 OCA], [https://pdbe.org/5uz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uz9 RCSB], [https://www.ebi.ac.uk/pdbsum/5uz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref> 

==See Also==
*[[CRISPR subtype I-F|CRISPR subtype I-F]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
[[Category: Pseudomonas phage JBD30]]
[[Category: Pseudomonas virus D3112]]
[[Category: Bondy-Denomy J]]
[[Category: Carter J]]
[[Category: Chowdhury S]]
[[Category: Davidson AR]]
[[Category: Fischer ER]]
[[Category: Hoffmann C]]
[[Category: Jackson RN]]
[[Category: Lander GC]]
[[Category: Maxwell KL]]
[[Category: Nosaka L]]
[[Category: Rollins MF]]
[[Category: Wiedenheft B]]