View source for 5vhs


==Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle==
<SX load='5vhs' size='340' side='right' viewer='molstar' caption='[[5vhs]], [[Resolution|resolution]] 8.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vhs]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VHS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vhs OCA], [https://pdbe.org/5vhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vhs RCSB], [https://www.ebi.ac.uk/pdbsum/5vhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vhs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRS7_HUMAN PRS7_HUMAN] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.<ref>PMID:9295362</ref> 
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== Publication Abstract from PubMed ==
The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 A resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions. Remarkably, the p28-bound AAA ring does not form a channel in the free RP and spontaneously samples multiple "open" and "closed" topologies at the Rpt2-Rpt6 and Rpt3-Rpt4 interfaces. Our analysis suggests that p28 assists the proteolytic core particle to select a specific conformation of the ATPase ring for RP engagement and is released in a shoehorn-like fashion in the last step of the chaperone-mediated proteasome assembly.

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.,Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma YB, Ouyang Q, Finley D, Kirschner MW, Mao Y Mol Cell. 2017 Jul 20;67(2):322-333.e6. doi: 10.1016/j.molcel.2017.06.007. Epub, 2017 Jul 6. PMID:28689658<ref>PMID:28689658</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5vhs" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ankyrin 3D structures|Ankyrin 3D structures]]
*[[Proteasome 3D structures|Proteasome 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Chen S]]
[[Category: Dong Y]]
[[Category: Finley D]]
[[Category: Kirschner MW]]
[[Category: Lu Y]]
[[Category: Ma YB]]
[[Category: Mao Y]]
[[Category: Ouyang Q]]
[[Category: Sun S]]
[[Category: Wu J]]