View source for 5vu2


==Electron cryo-microscopy of "immature" Chikungunya VLP==
<SX load='5vu2' size='340' side='right' viewer='molstar' caption='[[5vu2]], [[Resolution|resolution]] 6.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vu2]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus_strain_Senegal_37997 Chikungunya virus strain Senegal 37997]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VU2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vu2 OCA], [https://pdbe.org/5vu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vu2 RCSB], [https://www.ebi.ac.uk/pdbsum/5vu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vu2 ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/A0A0H4CWF2_CHIKV A0A0H4CWF2_CHIKV] 
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== Publication Abstract from PubMed ==
Cleavage of the alphavirus precursor glycoprotein p62 into the E2 and E3 glycoproteins before assembly with the nucleocapsid is the key to producing fusion-competent mature spikes on alphaviruses. Here we present a cryo-EM, 6.8-A resolution structure of an "immature" Chikungunya virus in which the cleavage site has been mutated to inhibit proteolysis. The spikes in the immature virus have a larger radius and are less compact than in the mature virus. Furthermore, domains B on the E2 glycoproteins have less freedom of movement in the immature virus, keeping the fusion loops protected under domain B. In addition, the nucleocapsid of the immature virus is more compact than in the mature virus, protecting a conserved ribosome-binding site in the capsid protein from exposure. These differences suggest that the posttranslational processing of the spikes and nucleocapsid is necessary to produce infectious virus.

Structural studies of Chikungunya virus maturation.,Yap ML, Klose T, Urakami A, Hasan SS, Akahata W, Rossmann MG Proc Natl Acad Sci U S A. 2017 Dec 4. pii: 1713166114. doi:, 10.1073/pnas.1713166114. PMID:29203665<ref>PMID:29203665</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5vu2" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
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[[Category: Chikungunya virus strain Senegal 37997]]
[[Category: Large Structures]]
[[Category: Rossmann MG]]
[[Category: Yap ML]]