View source for 5wda


==Structure of the PulG pseudopilus==
<SX load='5wda' size='340' side='right' viewer='molstar' caption='[[5wda]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wda]] is a 25 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_oxytoca Klebsiella oxytoca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WDA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wda OCA], [https://pdbe.org/5wda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wda RCSB], [https://www.ebi.ac.uk/pdbsum/5wda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wda ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Many Gram-negative bacteria use type 2 secretion systems (T2SSs) to secrete proteins involved in virulence and adaptation. Transport of folded proteins via T2SS nanomachines requires the assembly of inner membrane-anchored fibres called pseudopili. Although efficient pseudopilus assembly is essential for protein secretion, structure-based functional analyses are required to unravel the mechanistic link between these processes. Here, we report an atomic model for a T2SS pseudopilus from Klebsiella oxytoca, obtained by fitting the NMR structure of its calcium-bound subunit PulG into the ~5-A-resolution cryo-electron microscopy reconstruction of assembled fibres. This structure reveals the comprehensive network of inter-subunit contacts and unexpected features, including a disordered central region of the PulG helical stem, and highly flexible C-terminal residues on the fibre surface. NMR, mutagenesis and functional analyses highlight the key role of calcium in PulG folding and stability. Fibre disassembly in the absence of calcium provides a basis for pseudopilus length control, essential for protein secretion, and supports the Archimedes screw model for the type 2 secretion mechanism.An atomic model for a type 2 secretion system pseudopilus from Klebsiella oxytoca reveals a comprehensive network of inter-subunit contacts while mutational and functional analyses highlight the role of calcium in PulG folding and stability.

Structure of the calcium-dependent type 2 secretion pseudopilus.,Lopez-Castilla A, Thomassin JL, Bardiaux B, Zheng W, Nivaskumar M, Yu X, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Nat Microbiol. 2017 Oct 9. doi: 10.1038/s41564-017-0041-2. PMID:28993624<ref>PMID:28993624</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5wda" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pseudopilin|Pseudopilin]]
== References ==
<references/>
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[[Category: Klebsiella oxytoca]]
[[Category: Large Structures]]
[[Category: Bardiaux B]]
[[Category: Egelman EH]]
[[Category: Francetic O]]
[[Category: Izadi-Pruneyre N]]
[[Category: Lopez-Castilla A]]
[[Category: Nilges M]]
[[Category: Nivaskumar M]]
[[Category: Thomassin JL]]
[[Category: Yu X]]
[[Category: Zheng W]]