View source for 5wfe


==Cas1-Cas2-IHF-DNA holo-complex==
<SX load='5wfe' size='340' side='right' viewer='molstar' caption='[[5wfe]], [[Resolution|resolution]] 3.64&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wfe]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12], [https://en.wikipedia.org/wiki/Escherichia_coli_S88 Escherichia coli S88] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WFE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.64&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wfe OCA], [https://pdbe.org/5wfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wfe RCSB], [https://www.ebi.ac.uk/pdbsum/5wfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wfe ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAS1_ECOLI CAS1_ECOLI] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas1 is thought to be involved in CRISPR adaptation, the first stage of CRISPR immunity. Might be involved in the addition/removal of CRISPR spacers. Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates. Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems.<ref>PMID:21255106</ref> 

==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Escherichia coli K-12]]
[[Category: Escherichia coli S88]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Doudna JA]]
[[Category: Liu JJ]]
[[Category: Nogales E]]
[[Category: Wright AV]]