View source for 5wje


==Crystal structure of Naa80 bound to a bisubstrate analogue==
<StructureSection load='5wje' size='340' side='right'caption='[[5wje]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WJE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.765&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMC:CARBOXYMETHYL+COENZYME+*A'>CMC</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wje OCA], [https://pdbe.org/5wje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wje RCSB], [https://www.ebi.ac.uk/pdbsum/5wje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wje ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NAA80_DROME NAA80_DROME] N-alpha-acetyltransferase that acetylates the amino terminal acidic residue of proteins devoid of initiator methionine (PubMed:29581307). Preferentially acts on proteins starting with Asp-Asp-Asp and Glu-Glu-Glu sequences (PubMed:29581307). In vitro, shows high activity towards N-terminal sequences starting with Met-Asp-Glu-Leu, Met-Glu-Glu-Glu and Met-Asp-Asp-Asp (PubMed:29581307).<ref>PMID:29581307</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N-terminal (Nt) acetylation is a major protein modification catalyzed by N-terminal acetyltransferases (NATs). Methionine acidic N termini, including actin, are cotranslationally Nt acetylated by NatB in all eukaryotes, but animal actins containing acidic N termini, are additionally posttranslationally Nt acetylated by NAA80. Actin Nt acetylation was found to regulate cytoskeletal dynamics and motility, thus making NAA80 a potential target for cell migration regulation. In this work, we developed potent and selective bisubstrate inhibitors for NAA80 and determined the crystal structure of NAA80 in complex with such an inhibitor, revealing that NAA80 adopts a fold similar to other NAT enzymes but with a more open substrate binding region. Furthermore, in contrast to most other NATs, the substrate specificity of NAA80 is mainly derived through interactions between the enzyme and the acidic amino acids at positions 2 and 3 of the actin substrate and not residues 1 and 2. A yeast model revealed that ectopic expression of NAA80 in a strain lacking NatB activity partially restored Nt acetylation of NatB substrates, including yeast actin. Thus, NAA80 holds intrinsic capacity to posttranslationally Nt acetylate NatB-type substrates in vivo. In sum, the presence of a dominant cotranslational NatB in all eukaryotes, the specific posttranslational actin methionine removal in animals, and finally, the unique structural features of NAA80 leave only the processed actins as in vivo substrates of NAA80. Together, this study reveals the molecular and cellular basis of NAA80 Nt acetylation and provides a scaffold for development of inhibitors for the regulation of cytoskeletal properties.

Structural determinants and cellular environment define processed actin as the sole substrate of the N-terminal acetyltransferase NAA80.,Goris M, Magin RS, Foyn H, Myklebust LM, Varland S, Ree R, Drazic A, Bhambra P, Stove SI, Baumann M, Haug BE, Marmorstein R, Arnesen T Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1719251115. doi:, 10.1073/pnas.1719251115. PMID:29581307<ref>PMID:29581307</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5wje" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Arnesen T]]
[[Category: Goris M]]
[[Category: Magin RS]]
[[Category: Marmorstein R]]