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==Crystal structure of the parainfluenza virus 5 nucleoprotein-phosphoprotein complex==
<StructureSection load='5wkn' size='340' side='right'caption='[[5wkn]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_virus_5_(strain_W3) Simian virus 5 (strain W3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WKN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.653&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wkn OCA], [https://pdbe.org/5wkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wkn RCSB], [https://www.ebi.ac.uk/pdbsum/5wkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wkn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NCAP_PIV5 NCAP_PIV5] Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
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== Publication Abstract from PubMed ==
Parainfluenza virus 5 (PIV5) belongs to the family Paramyxoviridae, which consists of enveloped viruses with a non-segmented negative strand RNA genome encapsidated by the nucleoprotein (N). Paramyxovirus replication is regulated by the phosphoprotein (P) through protein-protein interactions with N and the RNA polymerase (L). The chaperone activity of P is essential to maintain the unassembled RNA-free form of N in order to prevent non-specific RNA binding and premature N oligomerization. Here, we determined the crystal structure of unassembled PIV5 N in complex with a P peptide (N(0)P) derived from the N-terminus of P (P50) at 2.65 A. The PIV5 N(0)P consists of two domains: an N-terminal domain (NTD) and a C-terminal domain (CTD) separated by a hinge region. The cleft at the hinge region of RNA-bound PIV5 N was previously shown as an RNA binding site. The N(0)P structure shows that the P peptide binds to the CTD of N and extends towards the RNA binding site to inhibit N oligomerization and hence, RNA binding. Binding of P peptide also keeps the PIV5 N in the open form. A molecular dynamics (MD) analysis of both the open and closed forms of N shows the flexibility of the CTD and the preference of the N protein to be in an open conformation. The gradual opening of the hinge region, to release the RNA, was also observed. Together these results advance our knowledge of the conformational swapping of N required for the highly regulated paramyxovirus replication.ImportanceParamyxovirus replication is regulated by the interaction of P with N and L proteins. Here, we report the crystal structure of unassembled parainfluenza virus 5 (PIV5) N chaperoned with P peptide. Our results provide a detailed understanding of the binding of P to N. The conformational switching of N between closed and open forms during its initial interaction with P, as well as during RNA release, was analyzed. Our data also show the plasticity of CTD and the importance of domain movement for the conformational switching. The results improve our understanding of the mechanism of interchanging N conformations for RNA replication and release.

Structure of the paramyxovirus PIV5 nucleoprotein in complex with an amino-terminal peptide of the phosphoprotein.,Aggarwal M, Leser GP, Kors CA, Lamb RA J Virol. 2017 Dec 13. pii: JVI.01304-17. doi: 10.1128/JVI.01304-17. PMID:29237836<ref>PMID:29237836</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Aggarwal M]]
[[Category: Kors CA]]
[[Category: Lamb RA]]
[[Category: Leser GP]]