View source for 5wm5


==Crystal Structure of CahJ in Complex with 5-Methylsalicyl Adenylate==
<StructureSection load='5wm5' size='340' side='right'caption='[[5wm5]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wm5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_gandocaensis Streptomyces gandocaensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WM5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.797&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=B5Y:9-(5-O-{(S)-hydroxy[(2-hydroxy-5-methylbenzene-1-carbonyl)oxy]phosphoryl}-alpha-L-lyxofuranosyl)-9H-purin-6-amine'>B5Y</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm5 OCA], [https://pdbe.org/5wm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wm5 RCSB], [https://www.ebi.ac.uk/pdbsum/5wm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A140DJY3_9ACTN A0A140DJY3_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cahuitamycins are biofilm inhibitors biosynthesized by a convergent NRPS pathway. Previous genetic analysis indicated that a discrete enzyme, CahJ, serves as a gatekeeper for cahuitamycin structural diversification. Herein, the CahJ protein was probed structurally, functionally and through mutasynthesis. This analysis enabled production of a new cahuitamycin congener through targeted precursor incorporation.

A Defined and Flexible Pocket Explains Aryl Substrate Promiscuity by the Cahuitamycin Starter Unit Activating Enzyme CahJ.,Sherman DH, Tripathi A, Park SR, Sikkema A, Cho HJ, Wu J, Lee B, Xi C, Smith JL Chembiochem. 2018 May 9. doi: 10.1002/cbic.201800233. PMID:29742306<ref>PMID:29742306</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wm5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces gandocaensis]]
[[Category: Sikkema AP]]
[[Category: Smith JL]]