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==Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis==
<StructureSection load='5wos' size='340' side='right'caption='[[5wos]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wos]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canarypox_virus Canarypox virus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WOS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wos OCA], [https://pdbe.org/5wos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wos RCSB], [https://www.ebi.ac.uk/pdbsum/5wos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wos ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARBH_CNPV ARBH_CNPV] Plays a role in the inhibition of host apoptosis. Interacts with host proapoptotic factors BAK1 and BAX to supposedly prevent their activation.<ref>PMID:29053589</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Programmed cell death or apoptosis is an important component of host defense systems against viral infection. The B-cell lymphoma 2 (Bcl-2) proteins family is the main arbiter of mitochondrially mediated apoptosis, and viruses have evolved sequence and structural mimics of Bcl-2 to subvert premature host cell apoptosis in response to viral infection. The sequencing of the canarypox virus genome identified a putative pro-survival Bcl-2 protein, CNP058. However, a role in apoptosis inhibition for CNP058 has not been identified to date. Here, we report that CNP058 is able to bind several host cell pro-death Bcl-2 proteins, including Bak and Bax, as well as several BH3 only-proteins including Bim, Bid, Bmf, Noxa, Puma, and Hrk with high to moderate affinities. We then defined the structural basis for CNP058 binding to pro-death Bcl-2 proteins by determining the crystal structure of CNP058 bound to Bim BH3. CNP058 adopts the conserved Bcl-2 like fold observed in cellular pro-survival Bcl-2 proteins, and utilizes the canonical ligand binding groove to bind Bim BH3. We then demonstrate that CNP058 is a potent inhibitor of ultraviolet (UV) induced apoptosis in a cell culture model. Our findings suggest that CNP058 is a potent inhibitor of apoptosis that is able to bind to BH3 domain peptides from a broad range of pro-death Bcl-2 proteins, and may play a key role in countering premature host apoptosis.

Structural and Functional Insight into Canarypox Virus CNP058 Mediated Regulation of Apoptosis.,Anasir MI, Baxter AA, Poon IKH, Hulett MD, Kvansakul M Viruses. 2017 Oct 20;9(10). pii: E305. doi: 10.3390/v9100305. PMID:29053589<ref>PMID:29053589</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wos" style="background-color:#fffaf0;"></div>

==See Also==
*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Canarypox virus]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Anasir MI]]
[[Category: Kvansakul M]]