View source for 5wq8


==CryoEM structure of type II secretion system secretin GspD in Vibrio cholerae==
<SX load='5wq8' size='340' side='right' viewer='molstar' caption='[[5wq8]], [[Resolution|resolution]] 3.26&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wq8]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WQ8 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wq7|5wq7]], [[5wq9|5wq9]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsD, VC_2733 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq8 OCA], [http://pdbe.org/5wq8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq8 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq8 ProSAT]</span></td></tr>
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== Function ==
[[http://www.uniprot.org/uniprot/GSPD_VIBCH GSPD_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins (By similarity). Required for secretion of cholera toxin through the outer membrane. 
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== Publication Abstract from PubMed ==
The secretin GspD of the type II secretion system (T2SS) forms a channel across the outer membrane in Gram-negative bacteria to transport substrates from the periplasm to the extracellular milieu. The lack of an atomic-resolution structure of the GspD channel hinders the investigation of substrate translocation mechanism of T2SS. Here we report cryo-EM structures of two GspD channels ( approximately 1 MDa), from Escherichia coli K12 and Vibrio cholerae, at approximately 3 A resolution. The structures reveal a pentadecameric channel architecture, wherein three rings of GspD N domains form the periplasmic channel. The secretin domain constitutes a novel double beta-barrel channel, with at least 60 beta-strands in each barrel, and is stabilized by S domains. The outer membrane channel is sealed by beta-strand-enriched gates. On the basis of the partially open state captured, we proposed a detailed gate-opening mechanism. Our structures provide a structural basis for understanding the secretin superfamily and the mechanism of substrate translocation in T2SS.

Structural insights into the secretin translocation channel in the type II secretion system.,Yan Z, Yin M, Xu D, Zhu Y, Li X Nat Struct Mol Biol. 2017 Feb;24(2):177-183. doi: 10.1038/nsmb.3350. Epub 2017, Jan 9. PMID:28067918<ref>PMID:28067918</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5wq8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Vibch]]
[[Category: Li, X]]
[[Category: Yan, Z]]
[[Category: Yin, M]]
[[Category: C15 symmetry]]
[[Category: Protein transport]]
[[Category: Secretin]]
[[Category: T2ss]]