View source for 5wtg


==Crystal structure of the Fab fragment of anti-HAV antibody R10==
<StructureSection load='5wtg' size='340' side='right'caption='[[5wtg]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wtg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.907&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtg OCA], [https://pdbe.org/5wtg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtg RCSB], [https://www.ebi.ac.uk/pdbsum/5wtg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtg ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hepatitis A virus (HAV) infects approximately 1.4 million people annually and, although there is a vaccine, there are no licensed therapeutic drugs. HAV is unusually stable (making disinfection problematic) and little is known of how it enters cells and releases its RNA. Here we report a potent HAV-specific monoclonal antibody, R10, which neutralizes HAV infection by blocking attachment to the host cell. High-resolution cryo-EM structures of HAV full and empty particles and of the complex of HAV with R10 Fab reveal the atomic details of antibody binding and point to a receptor recognition site at the pentamer interface. These results, together with our observation that the R10 Fab destabilizes the capsid, suggest the use of a receptor mimic mechanism to neutralize virus infection, providing new opportunities for therapeutic intervention.

Potent neutralization of hepatitis A virus reveals a receptor mimic mechanism and the receptor recognition site.,Wang X, Zhu L, Dang M, Hu Z, Gao Q, Yuan S, Sun Y, Zhang B, Ren J, Kotecha A, Walter TS, Wang J, Fry EE, Stuart DI, Rao Z Proc Natl Acad Sci U S A. 2017 Jan 10. pii: 201616502. doi:, 10.1073/pnas.1616502114. PMID:28074040<ref>PMID:28074040</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wtg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Dang M]]
[[Category: Fry EE]]
[[Category: Gao Q]]
[[Category: Hu Z]]
[[Category: Rao Z]]
[[Category: Ren J]]
[[Category: Stuart DI]]
[[Category: Sun Y]]
[[Category: Walter TS]]
[[Category: Wang J]]
[[Category: Wang X]]
[[Category: Yuan S]]
[[Category: Zhang B]]
[[Category: Zhu L]]