View source for 5wtl


==Crystal structure of the periplasmic portion of outer membrane protein A (OmpA) from Capnocytophaga gingivalis==
<StructureSection load='5wtl' size='340' side='right'caption='[[5wtl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wtl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Capnocytophaga_gingivalis_ATCC_33624 Capnocytophaga gingivalis ATCC 33624]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.298&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtl OCA], [https://pdbe.org/5wtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtl RCSB], [https://www.ebi.ac.uk/pdbsum/5wtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C2M2E7_CAPGI C2M2E7_CAPGI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Eukaryotic thrombospondin type 3 repeat (TT3R) is an efficient calcium ion (Ca(2+)) binding motif only found in mammalian thrombospondin family. TT3R has also been found in prokaryotic cellulase Cel5G, which was thought to forfeit the Ca(2+)-binding capability due to the formation of intra-repeat disulfide bonds, instead of the inter-repeat ones possessed by eukaryotic TT3Rs. In this study, we have identified an enormous number of prokaryotic TT3R-containing proteins belonging to several different protein families, including outer membrane protein A (OmpA), an important structural protein connecting the outer membrane and the periplasmic peptidoglycan layer in gram-negative bacteria. Here, we report the crystal structure of the periplasmic region of OmpA from Capnocytophaga gingivalis, which contains a linker region comprising five consecutive TT3Rs. The structure of OmpA-TT3R exhibits a well-ordered architecture organized around two tightly-coordinated Ca(2+) and confirms the presence of abnormal intra-repeat disulfide bonds. Further mutagenesis studies showed that the Ca(2+)-binding capability of OmpA-TT3R is indeed dependent on the proper formation of intra-repeat disulfide bonds, which help to fix a conserved glycine residue at its proper position for Ca(2+) coordination. Additionally, despite lacking inter-repeat disulfide bonds, the interfaces between adjacent OmpA-TT3Rs are enhanced by both hydrophobic and conserved aromatic-proline interactions.

Structure of thrombospondin type 3 repeats in bacterial outer membrane protein A reveals its intra-repeat disulfide bond-dependent calcium-binding capability.,Dai S, Sun C, Tan K, Ye S, Zhang R Cell Calcium. 2017 Sep;66:78-89. doi: 10.1016/j.ceca.2017.05.016. Epub 2017 Jun, 9. PMID:28807152<ref>PMID:28807152</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wtl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Capnocytophaga gingivalis ATCC 33624]]
[[Category: Large Structures]]
[[Category: Dai S]]
[[Category: Tan K]]
[[Category: Ye S]]
[[Category: Zhang R]]