View source for 5wv8


==Crystal structure of a mutant insect group III chitinase (CAD1-E217L) from Ostrinia furnacalis==
<StructureSection load='5wv8' size='340' side='right'caption='[[5wv8]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ostrinia_furnacalis Ostrinia furnacalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WV8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.042&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wv8 OCA], [https://pdbe.org/5wv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wv8 RCSB], [https://www.ebi.ac.uk/pdbsum/5wv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wv8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/U5LUV7_OSTFU U5LUV7_OSTFU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The glycoside hydrolase family 18 chitinases degrade or alter chitin. Multiple catalytic domains in a glycoside hydrolase family 18 chitinase function synergistically during chitin degradation. Here, an insect group III chitinase from the agricultural pest Ostrinia furnacalis (OfChtIII) is revealed to be an arthropod-conserved chitinase that contains two nonsynergistic GH18 domains according to its catalytic properties. Both GH18 domains are active towards single-chained chitin substrates, but are inactive towards insoluble chitin substrates. The crystal structures of each unbound GH18 domain, as well as of GH18 domains complexed with hexa-N-acetyl-chitohexaose or penta-N-acetyl-chitopentaose, suggest that the two GH18 domains possess endo-specific activities. Physiological data indicated that the developmental stage-dependent gene-expression pattern of OfChtIII was the same as that of the chitin synthase OfChsA but significantly different from that of the chitinase OfChtI, which is indispensable for cuticular chitin degradation. Additionally, immunological staining indicated that OfChtIII was co-localized with OfChsA. Thus, OfChtIII is most likely to be involved in the chitin-synthesis pathway.

The deduced role of a chitinase containing two nonsynergistic catalytic domains.,Liu T, Zhu W, Wang J, Zhou Y, Duan Y, Qu M, Yang Q Acta Crystallogr D Struct Biol. 2018 Jan 1;74(Pt 1):30-40. doi:, 10.1107/S2059798317018289. Epub 2018 Jan 1. PMID:29372897<ref>PMID:29372897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wv8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Chitinase 3D structures|Chitinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ostrinia furnacalis]]
[[Category: Liu T]]
[[Category: Yang Q]]
[[Category: Zhou Y]]