View source for 5wyb


==Structure of Pseudomonas aeruginosa DspI==
<StructureSection load='5wyb' size='340' side='right'caption='[[5wyb]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wyb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA14 Pseudomonas aeruginosa PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WYB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wyb OCA], [https://pdbe.org/5wyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wyb RCSB], [https://www.ebi.ac.uk/pdbsum/5wyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wyb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9I5I4_PSEAE Q9I5I4_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.

Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.,Liu L, Li T, Cheng XJ, Peng CT, Li CC, He LH, Ju SM, Wang NY, Ye TH, Lian M, Xiao QJ, Song YJ, Zhu YB, Yu LT, Wang ZL, Bao R Sci Rep. 2018 Mar 2;8(1):3928. doi: 10.1038/s41598-018-22300-1. PMID:29500457<ref>PMID:29500457</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wyb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PA14]]
[[Category: Bao R]]
[[Category: He L]]
[[Category: Li C]]
[[Category: Li T]]
[[Category: Liu L]]
[[Category: Peng C]]
[[Category: Shen Y]]
[[Category: Song Y]]
[[Category: Zhu Y]]